Rabbit Recombinant Monoclonal Cyclin B1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - PE Anti-Cyclin B1 antibody [Y106] (AB210615), expandable thumbnail](https://content.abcam.com/products/images/pe-cyclin-b1-antibody-y106-ab210615--flow-cytometry-intracellular-img26812.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-Cyclin B1 antibody [Y106] (AB210615)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes The cellular localisation of this product has been verified in ICC/IF |
Associated Products
Select an associated product type
11 products for Alternative Product
3 products for Alternative Version
Target data
Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Alternative names
CCNB, CCNB1, G2/mitotic-specific cyclin-B1
Recommended products
Rabbit Recombinant Monoclonal Cyclin B1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Y106
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not cross-react with other cyclin family members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-Cyclin B1 antibody [Y106] (ab210615), expandable thumbnail](https://content.abcam.com/products/images/pe-cyclin-b1-antibody-y106-ab210615--flow-cytometry-intracellular-img26812.jpg "Flow Cytometry (Intracellular) - PE Anti-Cyclin B1 antibody [Y106] (ab210615)")
Flow Cytometry (Intracellular) - PE Anti-Cyclin B1 antibody [Y106] (ab210615)
Overlay histogram showing HeLa cells stained with ab210615 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210615, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Downloads
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