AB223958

PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cytochrome P450 17A1/CYP17A1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CYP17, S17AH, CYP17A1, 17-alpha-hydroxyprogesterone aldolase, CYPXVII, Cytochrome P450 17A1, Cytochrome P450-C17, Steroid 17-alpha-monooxygenase, Cytochrome P450c17

1 Images

Flow Cytometry (Intracellular) - PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB223958)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB223958)

Overlay histogram showing HeLa cells stained with ab223958 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223958, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Carrier free

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - Low endotoxin, Azide free
Carrier free

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - BSA and Azide free
Unconjugated

Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
HRP

HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6293

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 17A1 also known as CYP17A1 is an essential enzyme in the steroidogenic pathway. Its molecular mass is approximately 57 kDa. It is part of the cytochrome P450 family and shows expression primarily in the adrenal glands and gonads. This enzyme catalyzes two key reactions: the 17α-hydroxylation of pregnenolone and progesterone and the subsequent 1720-lyase reaction. These reactions are critical for the production of steroid hormones such as glucocorticoids and sex steroids.

Biological function summary

CYP17A1 plays a central role in hormone biosynthesis. The enzyme exists as a single protein and does not form part of a larger complex. By facilitating the conversion of progestogens into androgens it directly impacts the balance and levels of steroid hormones in the body. This function supports vital processes like the development of secondary sexual characteristics and the regulation of metabolism therefore affecting multiple physiological functions.

Pathways

CYP17A1 integrates into both the steroidogenesis and biosynthesis pathways of adrenal and sex hormones. It links directly with proteins such as steroidogenic acute regulatory protein (StAR) and cytochrome P450 oxidoreductase (POR) which assist in enabling its enzymatic function. These pathways regulate the levels of hormones influencing biological processes including stress responses and reproductive functions.

CYP17A1 is associated with conditions like congenital adrenal hyperplasia and prostate cancer. In congenital adrenal hyperplasia CYP17A1 mutations disrupt the steroidogenesis pathway leading to cortisol production deficiency. In prostate cancer CYP17A1 is often upregulated contributing to increased androgen levels that fuel cancer progression. Proteins such as 3β-hydroxysteroid dehydrogenase (3β-HSD) and androgen receptor (AR) interact with CYP17A1 in these disorders demonstrating its importance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed : 22266943, PubMed : 25301938, PubMed : 27339894, PubMed : 9452426). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable) (PubMed : 25301938, PubMed : 9452426). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed : 22266943, PubMed : 25301938, PubMed : 27339894, PubMed : 36640554, PubMed : 9452426). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA (PubMed : 36640554). Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed : 25301938, PubMed : 27339894). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 22266943, PubMed : 25301938, PubMed : 27339894, PubMed : 9452426).

See full target information CYP17A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 23:e53065 PubMed36215690

2022

Lipid and protein content profiling of isolated native autophagic vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schmitt,Süleyman Bozkurt,Pascale Henning-Domres,Heike Huesmann,Stefan Eimer,Laura Bindila,Christian Behrends,Emily Boyle,Florian Wilfling,Georg Tascher,Christian Münch,Christian Behl,Andreas Kern

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]

Flow Cytometry (Intracellular) - PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB223958)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise