PE Anti-Cytokeratin 19 antibody [EPR1579Y]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-Cytokeratin 19 antibody [EPR1579Y] (AB216705)

Immunohistochemical analysis of formalin fixed paraffin embedded human gastric cancer labelling Cytokeratin 19 with ab216705 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Positive staining on human gastric cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-Cytokeratin 19 antibody [EPR1579Y] (AB216705)

Immunohistochemical analysis of formalin fixed paraffin embedded human glioma labelling Cytokeratin 19 with ab216705 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Negative control : no staining on human glioma.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-Cytokeratin 19 antibody [EPR1579Y] (AB216705)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung cancer labelling Cytokeratin 19 with ab216705 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Positive staining on human lung cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-Cytokeratin 19 antibody [EPR1579Y] (AB216705)

Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas labelling Cytokeratin 19 with ab216705 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Positive staining on human pancreas.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 19 antibody [EPR1579Y] (AB216705)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) labelling Cytokeratin 19 with ab216705 at 1/2000 dilution followed by ab150113 at 1/1000 (2μg/ml). Cells were counterstained with ab7291 at 1/1000 (1μg/ml) observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100.

Negative control : Jurkat.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 19 antibody [EPR1579Y] (AB216705)

Overlay histogram showing MCF7 cells stained with ab216705 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216705, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 BP bandpass filter.

This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.