Anti-Cytokeratin 5 [EP1601Y] PE conjugated antibody (ab224985) rabbit monoclonal antibody that is used to detect Cytokeratin 5 in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 5 antibody [EP1601Y] (AB224985), expandable thumbnail](https://content.abcam.com/products/images/pe-cytokeratin-5-antibody-ep1601y-ab224985--flow-cytometry-intracellular-img80721.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 5 antibody [EP1601Y] (AB224985)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes The cellular localisation of this product has been verified in ICC/IF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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5 products for Alternative Version
Target data
Function
Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Alternative names
58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5
Recommended products
Anti-Cytokeratin 5 [EP1601Y] PE conjugated antibody (ab224985) rabbit monoclonal antibody that is used to detect Cytokeratin 5 in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1601Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze, Store in the dark
Notes
PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.
Related products
Antibody clone EP1601Y is also available pre-conjugated to a variety of labels for your convenience – Anti-Cytokeratin 5 PE [EP1601Y] (ab224985).
Other related products
We have a range of other formats of antibody clone [EP1601Y] also available for your convenience:
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker ab52635, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y] ab193894, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] ab193895, HRP - HRP Anti-Cytokeratin 5 antibody [EP1601Y] ab193896, Carrier free - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free ab214586, APC - APC Anti-Cytokeratin 5 antibody [EP1601Y] ab224984, PE - ab224985, Anti-Cytokeratin 5 - Cytoskeleton Marker [EP1601Y] – Goat IgG (Chimeric) ab321868
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985), expandable thumbnail](https://content.abcam.com/products/images/pe-cytokeratin-5-antibody-ep1601y-ab224985--flow-cytometry-intracellular-img80721.jpg "Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)")
Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)
Overlay histogram showing A431 cells stained with ab224985 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224985, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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