AB210628

PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

RabMAb
Recombinant
Lab Essentials
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody - conjugated to PE. Cytoskeleton marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7

2 Images

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB210628)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB210628)

Figure Legend for the image : ab210628 staining Cytokeratin 7 in T74D cells. The cells were fixed with 100%methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210628 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in T74D cells fixed with 4%formaldehyde.

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB210628)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB210628)

Overlay histogram showing T47D cells stained with ab210628 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210628, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
Unconjugated

Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1619Y

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in T47D cells fixed with 100% methanol (5 min), and 4% formaldehyde (10 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303946 PubMed37897313

2023

Impaired Efferocytosis Enables Apoptotic Osteoblasts to Escape Osteoimmune Surveillance During Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Rongyao Xu,Hanyu Xie,Xin Shen,Jiadong Huang,Hengguo Zhang,Yu Fu,Ping Zhang,Songsong Guo,Dongmiao Wang,Sheng Li,Kai Zheng,Wen Sun,Laikui Liu,Jie Cheng,Hongbing Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB210628)

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB210628)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise