AB209297

PE Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

2 Images

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

Overlay histogram showing HeLa cells stained with ab209297 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209297, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

ab209297 staining Cytokeratin 8 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209297 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Unconjugated

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]
HRP

HRP Anti-Cytokeratin 8 antibody [EP1628Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1628Y

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478'>ab209478</a> - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Current oncology (Toronto, Ont.) 31:5677-5693 PubMed39330049

2024

A Preliminary Analysis of Circulating Tumor Microemboli from Breast Cancer Patients during Follow-Up Visits.

Applications

Unspecified application

Species

Unspecified reactive species

Hung-Chih Lai,Hsing-Hua Huang,Yun-Jie Hao,Hsin-Ling Lee,Chiao-Chan Wang,Thai-Yen Ling,Jen-Kuei Wu,Fan-Gang Tseng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Cytokeratin 8 antibody [EP1628Y]

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise