AB72469

PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Name: PE conjugated Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469) | Abcam
Brand: Abcam Limited
SKU: AB72469
Price: 585 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(10 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] - PE conjugated (ab72469) is a mouse monoclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in Flow Cytometry, ELISA.

- Trusted since 2008

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

2 Images

Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

Flow cytometry analysis of a recombinant (N-terminus DDDDK-tag) transmembrane cell surface receptor in CHO cells using ab72469 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

sELISA

Unknown

Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated DYKDDDDK tagged peptide and read after a 2 hour incubation at room temperature with ab72469.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Applications

sELISA, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), ELISA. Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone M2 is also available pre-conjugated to a variety of labels for your convenience – Anti-DDDDK tag (Binds to FLAG® tag sequence) PE [M2] (ab72469).

Other related products
We have a range of other formats of antibody clone [M2] also available for your convenience: Alkaline Phosphatase - ab81655, DyLight® 650 - ab117492, DyLight® 550 - ab117495, Carrier free - ab117505, PerCP - ab117514, Europium - ab187914

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.

Storage buffer

Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 638:1054-1064 PubMed39662508

2024

Discovery and engineering of the antibody response to a prominent skin commensal.

Applications

Unspecified application

Species

Unspecified reactive species

Djenet Bousbaine,Katherine D Bauman,Y Erin Chen,Pranav V Lalgudi,Tam T D Nguyen,Joyce M Swenson,Victor K Yu,Eunice Tsang,Sean Conlan,David B Li,Amina Jbara,Aishan Zhao,Arash Naziripour,Alessandra Veinbachs,Yu E Lee,Jennie L Phung,Alex Dimas,Sunit Jain,Xiandong Meng,Thi Phuong Thao Pham,Martin I McLaughlin,Layla J Barkal,Inta Gribonika,Koen K A Van Rompay,Heidi H Kong,Julia A Segre,Yasmine Belkaid,Christopher O Barnes,Michael A Fischbach

Cell 186:3307-3324.e30 PubMed37385249

2023

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Joleen S Cheah,Charles Xu,James Messing,Brian D Freibaum,Steven Boeynaems,J Paul Taylor,Namrata D Udeshi,Steven A Carr,Alice Y Ting

Molecular psychiatry 27:907-917 PubMed34980886

2022

Structure-function relationships of the disease-linked A218T oxytocin receptor variant.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Meyer,Benjamin Jurek,Mercedes Alfonso-Prieto,Rui Ribeiro,Vladimir M Milenkovic,Julia Winter,Petra Hoffmann,Christian H Wetzel,Alejandro Giorgetti,Paolo Carloni,Inga D Neumann

Synthetic biology (Oxford, England) 5:ysaa017 PubMed33392392

2020

Elucidation and refinement of synthetic receptor mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Hailey I Edelstein,Patrick S Donahue,Joseph J Muldoon,Anthony K Kang,Taylor B Dolberg,Lauren M Battaglia,Everett R Allchin,Mihe Hong,Joshua N Leonard

Genome research 28:1372-1382 PubMed29914970

2018

Genome-scale identification of cellular pathways required for cell surface recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Sumana Sharma,S Josefin Bartholdson,Amalie C M Couch,Kosuke Yusa,Gavin J Wright

Proceedings of the National Academy of Sciences of the United States of America 114:11392-11397 PubMed29073062

2017

Small-molecule inhibitors directly target CARD9 and mimic its protective variant in inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Elizaveta S Leshchiner,Jason S Rush,Michael A Durney,Zhifang Cao,Vlado Dančík,Benjamin Chittick,Huixian Wu,Adam Petrone,Joshua A Bittker,Andrew Phillips,Jose R Perez,Alykhan F Shamji,Virendar K Kaushik,Mark J Daly,Daniel B Graham,Stuart L Schreiber,Ramnik J Xavier

Journal of virology 91: PubMed28053110

2017

Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion.

Applications

Flow Cyt

Species

Unspecified reactive species

Salim M AlHajri,Cristina W Cunha,Anthony V Nicola,Hector C Aguilar,Hong Li,Naomi S Taus

EMBO reports 17:1657-1671 PubMed27601221

2016

Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Alex A Compton,Nicolas Roy,Françoise Porrot,Anne Billet,Nicoletta Casartelli,Jacob S Yount,Chen Liang,Olivier Schwartz

Journal of immunology (Baltimore, Md. : 1950) 189:4338-48 PubMed23041569

2012

Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastien M Maloveste,Dan Chen,Emma Gostick,Julian P Vivian,Ronald J Plishka,Ranjini Iyengar,Robin L Kruthers,Alicia Buckler-White,Andrew G Brooks,Jamie Rossjohn,David A Price,Bernard A P Lafont

PloS one 7:e44052 PubMed22937150

2012

Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells.

Applications

Flow Cyt

Species

Unspecified reactive species

Linda Raphel,Amarnath Talasila,Christine Cheung,Sanjay Sinha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (10)

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