Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to PE. Suitable for Flow Cyt, sELISA and reacts with Tag samples. Cited in 8 publications.
Images
![Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469), expandable thumbnail](https://content.abcam.com/products/images/pe-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72469--flow-cytometry-img63429.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)")
![Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469), expandable thumbnail](https://content.abcam.com/products/images/pe-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72469--sandwich-elisa-img24477.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72469)")
Publications
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- Molecular psychiatry 27:907-9172022Structure-function relationships of the disease-linked A218T oxytocin receptor variant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Meyer,Benjamin Jurek,Mercedes Alfonso-Prieto,Rui Ribeiro,Vladimir M Milenkovic,Julia Winter,Petra Hoffmann,Christian H Wetzel,Alejandro Giorgetti,Paolo Carloni,Inga D NeumannPubMed 34980886
- Synthetic biology (Oxford, England) 5:ysaa0172020Elucidation and refinement of synthetic receptor mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailey I Edelstein et. al.PubMed 33392392
- Genome research 28:1372-13822018Genome-scale identification of cellular pathways required for cell surface recognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumana Sharma et. al.PubMed 29914970
- Proceedings of the National Academy of Sciences of the United States of America 114:11392-113972017Small-molecule inhibitors directly target CARD9 and mimic its protective variant in inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizaveta S Leshchiner,Jason S Rush,Michael A Durney,Zhifang Cao,Vlado Dančík,Benjamin Chittick,Huixian Wu,Adam Petrone,Joshua A Bittker,Andrew Phillips,Jose R Perez,Alykhan F Shamji,Virendar K Kaushik,Mark J Daly,Daniel B Graham,Stuart L Schreiber,Ramnik J XavierPubMed 29073062
- Journal of virology 91:2017Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion.Applications:Flow CytReactive species:Unspecified reactive speciesSalim M AlHajri,Cristina W Cunha,Anthony V Nicola,Hector C Aguilar,Hong Li,Naomi S TausPubMed 28053110
- EMBO reports 17:1657-16712016Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex A Compton et. al.PubMed 27601221
- Journal of immunology (Baltimore, Md. : 1950) 189:4338-482012Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastien M Maloveste,Dan Chen,Emma Gostick,Julian P Vivian,Ronald J Plishka,Ranjini Iyengar,Robin L Kruthers,Alicia Buckler-White,Andrew G Brooks,Jamie Rossjohn,David A Price,Bernard A P LafontPubMed 23041569
- PloS one 7:e440522012Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells.Applications:Flow CytReactive species:Unspecified reactive speciesLinda Raphel et. al.PubMed 22937150
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Consider this alternative
Reactivity data
Select an application| Flow Cyt | sELISA | |
|---|---|---|
| Tag | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
8 products for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to PE. Suitable for Flow Cyt, sELISA and reacts with Tag samples. Cited in 8 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- M2
- Purification technique
- Size-exclusion chromatography
- Concentration
- Purification notes
Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469), expandable thumbnail](https://content.abcam.com/products/images/pe-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72469--flow-cytometry-img63429.jpg "Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)")
Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
Flow cytometry analysis of a recombinant (N-terminus DDDDK-tag) transmembrane cell surface receptor in CHO cells using ab72469 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.
![Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469), expandable thumbnail](https://content.abcam.com/products/images/pe-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72469--sandwich-elisa-img24477.jpg "Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)")
Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated DYKDDDDK tagged peptide and read after a 2 hour incubation at room temperature with ab72469.
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