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AB306229

PE Anti-DDX21 antibody [EPR14495]

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Rabbit Recombinant Monoclonal DDX21 antibody - conjugated to PE. Suitable for Antibody Labelling, Target Binding Affinity, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples.

View Alternative Names

Nucleolar RNA helicase 2, DEAD box protein 21, Gu-alpha, Nucleolar RNA helicase Gu, Nucleolar RNA helicase II, RH II/Gu, DDX21

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14495

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

ICC/IF, Target Binding Affinity, Antibody Labelling, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Proteins & Peptides

AB114574

Recombinant Human DDX6 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human DDX6 protein (GST tag N-Terminus) (AB114574)

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Primary Antibodies

AB109591

Anti-DDX55 antibody

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Primary Antibodies

AB307418

Anti-DDX6 antibody [EPR27016-10]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR27016-10] (AB307418)

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Primary Antibodies

AB126730

Anti-DDX5 antibody [EPR7239]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (AB126730)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX21 also known as RH-II/Gu is a member of the DEAD-box RNA helicase family. It weighs approximately 87 kDa and is expressed prominently in the nucleus. DEAD-box proteins are known for unwinding RNA secondary structures essential for various cellular processes. DDX21 plays an important role in ribosome biogenesis and transcriptional regulation. Its nuclear localization is critical for its function and it is detected in various cell types indicating a broad role in cellular mechanisms.
Biological function summary

DDX21 is involved in multiple fundamental cellular processes beyond just transcription and ribosomal RNA processing. It participates in the synthesis of rRNA and plays an active role in the regulation of ribosome assembly. Additionally DDX21 interacts with other members of the DEAD-box family forming a complex which is necessary for efficient RNA processing and modification. Its activity has influence over protein synthesis by regulating the availability of ribosomal components.

Pathways

DDX21 fits into the larger RNA processing and ribosome biogenesis pathways. It interacts with proteins like nucleolin and fibrillarin contributing to the assembly and maturation of ribosomes. In the ribosome biogenesis pathway DDX21 controls the processing of pre-rRNA together with other ribosomal proteins ensuring proper formation of functional ribosomes. DDX21 also participates in the stress response pathways indicating its integral role in adapting to cellular stress conditions.

DDX21's function is implicated in cancer and viral infections. High levels of DDX21 have been associated with tumorigenesis connecting it with oncogenic pathways. It interacts with proteins like p53 in the regulation of cellular growth and apoptosis. Additionally DDX21 plays a part in the replication of certain viruses suggesting a role in the viral life cycle and potential as a therapeutic target. Understanding DDX21's involvement in these conditions may offer insights into novel diagnostic and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II : promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed : 25470060, PubMed : 28790157). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs (PubMed : 25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (PubMed : 25470060, PubMed : 25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes : acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes (PubMed : 25470060). Functions as a cofactor for JUN-activated transcription : required for phosphorylation of JUN at 'Ser-77' (PubMed : 11823437, PubMed : 25260534). Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase) (PubMed : 9461305). Together with SIRT7, required to prevent R-loop-associated DNA damage and transcription-associated genomic instability : deacetylation by SIRT7 activates the helicase activity, thereby overcoming R-loop-mediated stalling of RNA polymerases (PubMed : 28790157). Involved in rRNA processing (PubMed : 14559904, PubMed : 18180292). May bind to specific miRNA hairpins (PubMed : 28431233). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1 (By similarity).
See full target information Nucleolar RNA helicase 2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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