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AB95619

PE Anti-DR5 antibody [MD5-1]

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(1 Publication)

Anti-DR5 antibody [MD5-1] conjugated to PE - suitable for flow cytometry, and reacts with mouse samples. Clone [MD5-1] is a Armenian Hamster monoclonal antibody cited in 7 publications.

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Flow Cytometry - PE Anti-DR5 antibody [MD5-1] (AB95619)
  • Flow Cyt

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Flow Cytometry - PE Anti-DR5 antibody [MD5-1] (AB95619)

Flow cytometry staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.125 µg of Golden Syrian Hamster IgG Isotype Control PE (open histogram) or 0.125 µg of ab95619 (filled histogram). Total viable cells were used for analysis.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

MD5-1

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Mouse Tnfrsf10b. The exact immunogen used to generate this antibody is proprietary information.

Q9QZM4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125-0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>Staining the cell sample in a final volume of 100 μl is recommended.</p><p><a href='/en-us/products/unavailable/pe-armenian-hamster-igg-monoclonal-isotype-control-ab101026'>ab101026</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DR5 also known as Death Receptor 5 TRAIL-R2 or TNFRSF10B is a protein critical in apoptosis. It is a transmembrane receptor with a mass of approximately 54 kDa. Researchers find it expressed mostly in tissues such as lung colon and prostate. DR5 functions by binding to its ligand TRAIL triggering the extrinsic apoptotic pathway. Through this mechanism DR5 initiates a cascade of caspase activation that leads to cell death.
Biological function summary

DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.

Pathways

DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.

DR5 exhibits significant relevance to cancer and autoimmune diseases. Its ability to induce apoptosis through the TRAIL pathway keeps oncogenic transformations in check. In cancer DR5 often interfaces with other proteins like Bcl-2 which are involved in cell survival. Furthermore defects or aberrant expressions in DR5 have associations with autoimmune disorders where excessive cell death or survival contributes to disease pathology. DR5's role in these conditions makes it a promising target in therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
See full target information Tnfrsf10b

Alternative Names

CD262, Dr5, Killer, Tnfrsf10b, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, MK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC urology 24:147 PubMed39014364

2024

m6A reader IGF2BP2 promotes M2 macrophage polarization and malignant biological behavior of bladder cancer by stabilizing NRP1 mRNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dian Fu,Xiuquan Shi,Xiaoming Yi,Ding Wu,Haowei He,Wenquan Zhou,Wen Cheng
View all publications
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Product promise

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