Rabbit Recombinant Monoclonal DSTN antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.
IgG
Rabbit
PE
Ex: 480;565nm, Em: 578nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Liquid
Monoclonal
Flow Cyt (Intra) | |
---|---|
Human | Predicted |
Mouse | Tested |
Rat | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes The cellular localisation of this product has been verified in ICC/IF. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Select an associated product type
Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
ACTDP, DSN, DSTN, Destrin, Actin-depolymerizing factor, ADF
Rabbit Recombinant Monoclonal DSTN antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.
IgG
Rabbit
PE
Ex: 480;565nm, Em: 578nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Liquid
Monoclonal
EPR15827(B)
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Upon delivery aliquot
Do Not Freeze, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
DSTN also known as destrin or actin depolymerizing factor is an actin-binding protein with a relatively low molecular mass of approximately 19 kDa. DSTN plays a significant role in actin filament dynamics by enhancing the disassembly of actin filaments into monomeric actin components thereby regulating cytoskeleton organization. The protein is present in a wide range of tissues including the brain and skeletal muscles indicating its importance in various physiological processes.
DSTN regulates actin cytoskeleton remodeling impacting cell shape and motility. It acts by binding to actin enhancing the turnover and dismantling of actin filaments which is critical for maintaining cellular architecture. DSTN is not known to be part of a larger protein complex but it interacts with other actin-related proteins to exert its function efficiently. Its role in cell movement and structure integrates it into larger cellular processes such as cell division migration and potentially signal transduction.
Cytoskeletal rearrangement and cell motility highlight the involvement of DSTN. DSTN associates closely with the Rho and Rac signaling pathways pivotal for cytoskeletal changes and cell division processes. Through these pathways DSTN contributes to modulating cellular responses by interacting with proteins like cofilin another actin-binding protein. This interaction with signaling pathways highlights DSTN's broader influence on cellular functions.
DSTN has an association with neurodegenerative diseases and cancer progression. Aberrations in its activity can result in cytoskeletal dysfunctions to which disorders such as Alzheimer’s disease relate due to the importance of cytoskeletal integrity in neuron function. Additionally DSTN's involvement in cell motility links it to cancer metastasis where unchecked cell movement promotes tumor spread. In these diseases DSTN collaborates mutably with the tau protein and other cytoskeletal regulators highlighting DSTN's relevance in pathological conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Overlay histogram showing NIH3T3 cells stained with ab213356 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213356, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in NIH3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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