AB239330

PE/DyLight™ 594 Anti-CD13 antibody [WM15]

Name: Anti-CD13 PE/DyLight™ 594 antibody [WM15] (ab239330) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB239330
Price: 360 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CD13 antibody - conjugated to PE/DyLight™ 594. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing ANPEP protein.

View Alternative Names

CD13, APN, PEPN, ANPEP, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, AP-M

1 Images

Flow Cytometry - PE/DyLight™ 594 Anti-CD13 antibody [WM15] (AB239330)

Flow Cyt

Supplier Data

Flow Cytometry - PE/DyLight™ 594 Anti-CD13 antibody [WM15] (AB239330)

Flow cytometric analysis of human peripheral blood leukocytes labeling CD13 with ab239330. Surface staining. Gated on leukocytes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WM15

Isotype

IgG1

Conjugation

PE/DyLight™ 594

Excitation/Emission

Ex: 565nm, Em: 618nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ANPEP protein. The exact immunogen used to generate this antibody is proprietary information.

P15144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibody is conjugated with tandem dye PE-DyLight™ 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.

Biological function summary

CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.

Pathways

CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.

Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).. (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.. (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.

See full target information ANPEP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39200219

2024

The Protective Role of Troxerutin (Trox) in Counteracting Anaplastic Thyroid Carcinoma (ATC) Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Bova,Rossella Basilotta,Giovanna Casili,Marika Lanza,Alessia Filippone,Michela Campolo,Anna Paola Capra,Giulia Vitale,Giulia Chisari,Cristina Colarossi,Dario Giuffrida,Irene Paterniti,Emanuela Esposito

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/DyLight™ 594 Anti-CD13 antibody [WM15]

Flow Cytometry - PE/DyLight™ 594 Anti-CD13 antibody [WM15] (AB239330)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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