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AB130738

PE Anti-EGFR antibody [EGFR1]

4

(1 Review)

|

(5 Publications)

Mouse Monoclonal EGFR antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human, Horse samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing EGFR protein.

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

1 Images
Flow Cytometry - PE Anti-EGFR antibody [EGFR1] (AB130738)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-EGFR antibody [EGFR1] (AB130738)

Analysis by flow cytometry of ab130738 staining EGFR in A431 cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EGFR1

Isotype

IgG2b

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Horse, Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing EGFR protein. The exact immunogen used to generate this antibody is proprietary information.

P00533

Epitope

Within amino acids 6-273

Specificity

ab130738 reacts with the extracellular domain of Human EGFR.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100 μl of whole blood.</p><p><a href='/en-us/products/primary-antibodies/pe-mouse-igg2b-plpv219-isotype-control-ab91532'>ab91532</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100 μl of whole blood.</p><p><a href='/en-us/products/primary-antibodies/pe-mouse-igg2b-plpv219-isotype-control-ab91532'>ab91532</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: 99% PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
See full target information EGFR
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:101726 PubMed35157848

2022

ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Tejeshwar C Rao,Reena R Beggs,Katherine E Ankenbauer,Jihye Hwang,Victor Pui-Yan Ma,Khalid Salaita,Susan L Bellis,Alexa L Mattheyses

PloS one 3:e1936 PubMed18398462

2008

Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Johan Rebetz,Dongping Tian,Annette Persson,Bengt Widegren,Leif G Salford,Elisabet Englund,David Gisselsson,Xiaolong Fan

Journal of cell science 116:4043-52 PubMed12953061

2003

Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas O Fortunel,Jacques A Hatzfeld,Pierre-Antoine Rosemary,Corinne Ferraris,Marie-Noëlle Monier,Valérie Haydont,Joanna Longuet,Benoit Brethon,Bing Lim,Isabelle Castiel,Rainer Schmidt,Antoinette Hatzfeld

Oncogene 20:2101-11 PubMed11360194

2001

Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

A Aigner,H Juhl,C Malerczyk,A Tkybusch,C C Benz,F Czubayko

The Journal of investigative dermatology 114:321-7 PubMed10651993

2000

Changing patterns of localization of putative stem cells in developing human hair follicles.

Applications

Unspecified application

Species

Unspecified reactive species

M Akiyama,L T Smith,H Shimizu
View all publications
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