AB27764

PE Anti-EGFR antibody [ICR10]

Name: Anti-EGFR PE antibody [ICR10] (ab27764) Rat monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB27764
Price: 435 USD
Availability: InStock

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(7 Publications)

Rat Monoclonal EGFR antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human EGFR.

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

1 Images

Flow Cytometry - PE Anti-EGFR antibody [ICR10] (AB27764)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-EGFR antibody [ICR10] (AB27764)

Left-Hand - isotope control; Right- Hand - ab27764 (dilution 1/10) staining A431 cells.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

ICR10

Isotype

IgG2a

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human EGFR.

P00533

Epitope

ab27764 binds to epitope B from EGFR and has an affinity of 6.7 x 10-9 M.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-pe-isotype-control-ab134674'>ab134674</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.

See full target information EGFR

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 10:19 PubMed29456764

2018

Integrative analysis of the epigenetic basis of muscle-invasive urothelial carcinoma.

Applications

Flow Cyt

Species

Unspecified reactive species

Thomas Sanford,Maxwell V Meng,Reema Railkar,Piyush K Agarwal,Sima P Porten

Bladder cancer (Amsterdam, Netherlands) 4:77-90 PubMed29430509

2018

Molecular Correlates of Responses to Dacomitinib and Afatinib in Bladder Cancer.

Applications

Flow Cyt

Species

Human

Shuzo Tamura,Yin Wang,Brendan Veeneman,Daniel Hovelson,Armand Bankhead,Luke J Broses,Guadalupe Lorenzatti Hiles,Monica Liebert,John R Rubin,Kathleen C Day,Maha Hussain,Nouri Neamati,Scott Tomlins,Philip L Palmbos,Petros Grivas,Mark L Day

Oncogene 34:2621-30 PubMed25043298

2014

Oncogenic role of Merlin/NF2 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

P A Guerrero,W Yin,L Camacho,D Marchetti

Blood 118:1255-63 PubMed21653320

2011

A transgene-encoded cell surface polypeptide for selection, in vivo tracking, and ablation of engineered cells.

Applications

Flow Cyt

Species

Human

Xiuli Wang,Wen-Chung Chang,ChingLam W Wong,David Colcher,Mark Sherman,Julie R Ostberg,Stephen J Forman,Stanley R Riddell,Michael C Jensen

Cancer letters 296:150-9 PubMed20444542

2010

Acquired resistance to cetuximab is mediated by increased PTEN instability and leads cross-resistance to gefitinib in HCC827 NSCLC cells.

Applications

Flow Cyt

Species

Human

Sun Mi Kim,Ji Su Kim,Joo-Hang Kim,Chae-Ok Yun,Eun Mi Kim,Hyun Ki Kim,Flavio Solca,Soo-Young Choi,Byoung Chul Cho

Stem cells and development 19:209-20 PubMed19799519

2009

Asymmetric distribution of epidermal growth factor receptor directs the fate of normal and cancer keratinocytes in vitro.

Applications

Flow Cyt

Species

Human

Hélène Le Roy,Thomas Zuliani,Isabelle Wolowczuk,Nathan Faivre,Nathalie Jouy,Bernadette Masselot,Jean-Pierre Kerkaert,Pierre Formstecher,Renata Polakowska

Cell biophysics 22:129-46 PubMed7534211

1993

Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor.

Applications

Unspecified application

Species

Unspecified reactive species

H Modjtahedi,S A Eccles,G Box,J Styles,C J Dean

View all publications

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