AB282791

PE Anti-EGR2 antibody [EPR23228-40]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal EGR2 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Egr-2, Krox-20, Zfp-25, Egr2, E3 SUMO-protein ligase EGR2, E3 SUMO-protein transferase ERG2, Early growth response protein 2, Zinc finger protein Krox-20, EGR-2

1 Images

Flow Cytometry (Intracellular) - PE Anti-EGR2 antibody [EPR23228-40] (AB282791)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-EGR2 antibody [EPR23228-40] (AB282791)

Flow cytometry staining of 2% paraformaldehyde fixed, True-Nuclear buffer™ permeabilized C57 BL/6 mouse splenocytes (top) or C57 BL/6 mouse splenocytes treated with ConA (2.5 μg/ml, 24 hours) (bottom), with ab282791 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left).

C57 BL/6 mouse splenocytes were incubated for 30 min in 1x PBS containing 10 μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab282791) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype control (1x 10⁶ cells in 100 μl) at 0.2 μg/ml for 30 min. The cells were simultaneously stained with CD4 APC.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live cells.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-EGR2 antibody [EPR23228-40]
Unconjugated

Anti-EGR2 antibody [EPR23228-40]
Carrier free

Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23228-40

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGR2 also known as early growth response 2 or KROX20 refers to a transcription factor with a mass of approximately 53 kDa. This protein plays a significant role in gene expression and regulates various genes involved in cellular growth and differentiation. EGR2 is expressed in various tissues with notable presence in the nervous system particularly within Schwann cells and the developing vertebrate nervous system. It can also be found in immune system cells including T and B cells.

Biological function summary

EGR2 influences the development and function of the peripheral nervous system contributing to processes like nerve myelination. It acts as a transcriptional regulator controlling the expression of genes that are essential for the maintenance of myelin sheaths around nerves. While EGR2 does not directly form part of a protein complex its role as a transcription factor allows it to interact with many other proteins to exert its regulatory functions.

Pathways

EGR2 participates in cellular pathways such as the MAPK/ERK signaling pathway and the immune response pathway. In the MAPK/ERK pathway EGR2 interacts with proteins like ERK1/2 to regulate cell growth and differentiation. In immune response pathways EGR2 modulates the differentiation of T cells impacting immune homeostasis and function by influencing gene expression pertinent to immune regulation.

Mutations or dysregulation of EGR2 can relate to Charcot-Marie-Tooth disease type 1 a peripheral nerve disorder. This neuropathy impacts myelin production where alterations in EGR2 play a role alongside proteins like PMP22 which are also affected in the disorder. Additionally EGR2 expression irregularities have been studied in connection with systemic lupus erythematosus in which immune cell regulation becomes impaired affecting proteins involved in immune signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific DNA-binding transcription factor (PubMed : 11823429, PubMed : 1674431, PubMed : 1969796, PubMed : 31852952). Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin (PubMed : 11823429, PubMed : 1674431, PubMed : 1969796, PubMed : 31852952, PubMed : 8093858). Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation (PubMed : 11823429). Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2 (PubMed : 17938205, PubMed : 1969796, PubMed : 8093858). Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres (PubMed : 11823429, PubMed : 1674431). Promotes the expression of HOXB3 in the rhombomere r5 and of HOXB3 in r3 and r5 in the hindbrain (PubMed : 11823429, PubMed : 8093858). Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells (PubMed : 10068633, PubMed : 7935840). Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons (PubMed : 11509834). May play a role in adipogenesis, possibly by regulating the expression of CEBPB (PubMed : 16054051).. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.

See full target information Egr2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-EGR2 antibody [EPR23228-40]

Flow Cytometry (Intracellular) - PE Anti-EGR2 antibody [EPR23228-40] (AB282791)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise