JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB278687

PE Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal eIF4EBP1 phospho T37 + T46 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1 phospho T37 + T46.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

1 Images
Flow Cytometry - PE Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278687)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278687)

Flow cytometric analysis of Jurkat cells unstained LY294002 treated cells as negative control (blue) or stained and treated with LY294002 (red) or with TPA (green) using ab278687.

  • Unconjugated

    Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

  • 660 APC

    APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

  • 519 FITC

    FITC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

4EB1T37T46-A5

Isotype

IgG

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human EIF4EBP1 phospho T37 + T46. The exact immunogen used to generate this antibody is proprietary information.

Q13541

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
See full target information EIF4EBP1 phospho T37 + T46
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:122 PubMed40158101

2025

The m6A reader IGF2BP2 promotes pancreatic cancer progression through the m6A-SLC1A5-mTORC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Pu,Yuting Wu,Weiguo Long,Xinyu Sun,Xiao Yuan,Deqiang Wang,Xu Wang,Min Xu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB278748

PE Anti-PTEN (phospho S380) antibody [PTENS380-NA9]

Recombinant

Flow Cytometry - PE Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (AB278748)

  • 0
  • 0
Primary Antibodies

AB278692

FITC Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

Recombinant

Flow Cytometry - FITC Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278692)

  • 0
  • 0
Primary Antibodies

AB60948

Anti-S6K1 (phospho T389 + T412) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T389 + T412) antibody (AB60948)

  • 1
  • 1
Primary Antibodies

AB109268

Anti-mTOR (phospho S2448) antibody [EPR426(2)]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

  • 4
  • 6

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com