AB90751

PE Anti-ENPP3/B10 antibody [NP4D6]

Name: Anti-ENPP3/B10 PE antibody [NP4D6] (ab90751) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB90751
Rating: 4 (3 reviews)

(3 Reviews)

(1 Publication)

Mouse Monoclonal ENPP3/B10 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing ENPP3 protein.

View Alternative Names

CD203c, PDNP3, ENPP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, NPP3, Alkaline phosphodiesterase I, Dinucleoside polyphosphatase, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I beta, Phosphodiesterase I/nucleotide pyrophosphatase 3, NPPase, PD-Ibeta

6 Images

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

ab90751 staining ENPP3/B10 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.

Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3/B10 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Blocking and diluting buffer : 5% NFDM/TBST We are not sure how to define the extra band below band of interest.

All lanes:

Western blot - Anti-SENP2 antibody [EPR4356(2)] (<a href='/en-us/products/primary-antibodies/senp2-antibody-epr43562-ab124724'>ab124724</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

MDA-MB-435S (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 4:

NIH: OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Exposure time: 40s

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Blocking and Diluting Buffer : 5% NFDM /TBST 87 kDa ProPC1/3; 66 kDa mature form (PMID : 26778167) Lane1 Expsoure Time : 20 seconds Lane2 and Lane3 were developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-PC1/3 antibody [EPR21908] (<a href='/en-us/products/primary-antibodies/pc1-3-antibody-epr21908-ab220363'>ab220363</a>) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg

Lane 2:

Mouse pancreas lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 66 kDa

false

Exposure time: 80s

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

IBA1 is a relatively minor protein of brain and is much more abundant in spleen (PMID : 8912632, PMID : 29232670). We suggest loading higher amount of brain lysate or using lower dilution of antibody for detecting signal in brain related lysates.

All lanes:

Western blot - Anti-Iba1 antibody [EPR16589] - Microglia marker (<a href='/en-us/products/primary-antibodies/iba1-antibody-epr16589-microglia-marker-ab178847'>ab178847</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse hippocampus tissue at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat hippocampus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

false

Exposure time: 40s

Supplier Data

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Blocking and diluting buffer : 5% NFDM/TBST Lysate/protein at 0.01 µg per lane.

All lanes:

Western blot - Anti-SENP2 antibody [EPR4356(2)] (<a href='/en-us/products/primary-antibodies/senp2-antibody-epr43562-ab124724'>ab124724</a>) at 1/10000 dilution

Secondary

All lanes:

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 46 kDa

false

Exposure time: 20s

Unconjugated

Anti-ENPP3/B10 antibody [NP4D6]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NP4D6

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, Flow Cyt (Intra), ICC/IF

applications

Immunogen

Cell preparation containing ENPP3 protein. The exact immunogen used to generate this antibody is proprietary information.

O14638

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "20 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.

Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed : 29717535, PubMed : 9344668). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well (PubMed : 12846830). In addition, displays an alkaline phosphodiesterase activity in vitro (PubMed : 11342463).

See full target information ENPP3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 17:141-149 PubMed32108036

2020

KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Kitagawa,Takaaki Masuda,Junichi Takahashi,Taro Tobo,Miwa Noda,Yosuke Kuroda,Qingjiang Hu,Yuta Kouyama,Yuta Kobayashi,Shotaro Kuramitsu,Kuniaki Sato,Atsushi Fujii,Yukihiro Yoshikawa,Hiroaki Wakiyama,Dai Shimizu,Yusuke Tsuruda,Hidetoshi Eguchi,Yuichiro Doki,Masaki Mori,Koshi Mimori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-ENPP3/B10 antibody [NP4D6]

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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