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Mouse Monoclonal ENPP3/B10 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing ENPP3 protein.


Images

Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751), expandable thumbnail
  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751), expandable thumbnail
  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751), expandable thumbnail
  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (AB90751), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing ENPP3 protein. The exact immunogen used to generate this antibody is proprietary information. Database link O14638

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected

Tested
Tested

Species
Human
Dilution info
20 µL for 106 Cells
Notes

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
20 µL for 106 Cells
Notes

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

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Target data

Function

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed:29717535, PubMed:9344668). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well (PubMed:12846830). In addition, displays an alkaline phosphodiesterase activity in vitro (PubMed:11342463).

Alternative names

CD203c, PDNP3, ENPP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, NPP3, Alkaline phosphodiesterase I, Dinucleoside polyphosphatase, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I beta, Phosphodiesterase I/nucleotide pyrophosphatase 3, NPPase, PD-Ibeta

Recommended products

Mouse Monoclonal ENPP3/B10 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing ENPP3 protein.

Key facts

Isotype
IgG1
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing ENPP3 protein. The exact immunogen used to generate this antibody is proprietary information. Database link O14638
Clone number
NP4D6
Purification technique
Size-exclusion chromatography
Concentration
Loading...
Purification notes

The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.

Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

Associated diseases and disorders

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.

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6 product images

  • Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751), expandable thumbnail

    Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)

    ab90751 staining ENPP3/B10 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)

    Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, FITC Anti-CD63 antibody [MEM-259] ab18235, green) and PE-conjugated anti-ENPP3/B10 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).

  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751), expandable thumbnail

    Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)

    Blocking and diluting buffer: 5% NFDM/TBST

    Lysate/protein at 0.01 µg per lane.

    All lanes: Western blot - Anti-SENP2 antibody [EPR4356(2)] (Anti-SENP2 antibody [EPR4356(2)] ab124724) at 1/10000 dilution

    Secondary

    All lanes: Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

    Predicted band size: 68 kDa

    Observed band size: 46 kDa

    Exposure time: 20s

  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751), expandable thumbnail

    Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)

    Blocking and Diluting Buffer: 5% NFDM /TBST

    87 kDa ProPC1/3; 66 kDa mature form (PMID: 26778167)

    Lane1 Expsoure Time: 20 seconds

    Lane2 and Lane3 were developed using a high sensitivity ECL substrate.

    All lanes: Western blot - Anti-PC1/3 antibody [EPR21908] (Anti-PC1/3 antibody [EPR21908] ab220363) at 1/1000 dilution

    Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg

    Lane 2: Mouse pancreas lysate at 20 µg

    Lane 3: Mouse brain lysate at 20 µg

    Secondary

    All lanes: Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

    Predicted band size: 87 kDa

    Observed band size: 66 kDa

    Exposure time: 80s

  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751), expandable thumbnail

    Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)

    Blocking and diluting buffer: 5% NFDM/TBST

    We are not sure how to define the extra band below band of interest.

    All lanes: Western blot - Anti-SENP2 antibody [EPR4356(2)] (Anti-SENP2 antibody [EPR4356(2)] ab124724) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: MDA-MB-435S (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

    Lane 4: NIH: OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

    Exposure time: 40s

  • Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751), expandable thumbnail

    Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    IBA1 is a relatively minor protein of brain and is much more abundant in spleen (PMID: 8912632, PMID: 29232670). We suggest loading higher amount of brain lysate or using lower dilution of antibody for detecting signal in brain related lysates.

    All lanes: Western blot - Anti-Iba1 antibody [EPR16588] (Anti-Iba1 antibody [EPR16588] ab178846) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Mouse hippocampus tissue at 20 µg

    Lane 4: Rat spleen tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Lane 6: Rat hippocampus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751) at 1/20000 dilution

    Predicted band size: 16 kDa

    Observed band size: 17 kDa

    Exposure time: 40s

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