JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB112068

PE Anti-EpCAM antibody [VU-1D9]

5

(1 Review)

|

(25 Publications)

Mouse Monoclonal EPCAM antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 25 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

1 Images
Flow Cytometry - PE Anti-EpCAM antibody [VU-1D9] (AB112068)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-EpCAM antibody [VU-1D9] (AB112068)

Flow cytometry analysis (surface staining) of human MCF-7 and SP2 cell lines with PE Anti-EpCAM antibody [VU-1D9] (ab112068)

  • Carrier free

    Anti-EpCAM antibody [VU-1D9] - BSA and Azide free

  • Unconjugated

    Anti-EpCAM antibody [VU-1D9]

  • 660 APC

    APC Anti-EpCAM antibody [VU-1D9]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EpCAM antibody [VU-1D9]

  • Biotin

    Biotin Anti-EpCAM antibody [VU-1D9]

  • 519 FITC

    FITC Anti-EpCAM antibody [VU-1D9]

  • 778 PE/Cy7®

    PE/Cy7® Anti-EpCAM antibody [VU-1D9]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

VU-1D9

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

Epitope

ab112068 recognizes an epitope within EGF-like domain I of EpCAM.

Specificity

Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.

style
left-column

Alternative Products

Primary Antibodies

AB213500

Anti-EpCAM antibody [EPR20532-222]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20532-222] (AB213500)

  • 4
  • 3
Primary Antibodies

AB124825

Anti-EpCAM antibody [EPR677(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

  • 1
  • 1
Primary Antibodies

AB225894

Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (AB225894)

  • 0
  • 0
Primary Antibodies

AB221552

Anti-EpCAM antibody [EPR20533-63]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] (AB221552)

  • 5
  • 4
Primary Antibodies

AB213501

Anti-EpCAM antibody [EPR20533-266]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] (AB213501)

  • 0
  • 0
Primary Antibodies

AB32392

Anti-EpCAM antibody [E144]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-EpCAM antibody [E144] (AB32392)

  • 4
  • 19
Primary Antibodies

AB223582

Anti-EpCAM antibody [EPR20532-225]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] (AB223582)

  • 5
  • 2

Complementary Products

Assay Kits

AB264632

Human EpCAM ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human EpCAM ELISA Kit (AB264632)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB200317

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

  • 0
  • 1
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM
style
left-column

Publications (25)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:144 PubMed40379789

2025

Multi-cancer detection of circulating tumor cells by targeting oncofetal chondroitin sulfate.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Løppke,Randi Ugleholdt,Christine F Secher,Nicolai T Sand,Joana Mujollari,Tobias Gustavsson,Robert Dagil,Thor G Theander,Ali Salanti,Kristoffer S Rohrberg,Mette Ø Agerbæk

Journal of ovarian research 17:52 PubMed38402185

2024

The miR-1290/OGN axis in ovarian cancer-associated fibroblasts modulates cancer cell proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Biyao Jiang,Songshu Xiao,Shan Zhang,Fang Xiao

ACS nano 17:18441-18455 PubMed37698887

2023

Drug-Loaded Lipid Magnetic Nanoparticles for Combined Local Hyperthermia and Chemotherapy against Glioblastoma Multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Lilianne Beola,Nerea Iturrioz-Rodríguez,Carlotta Pucci,Rosalia Bertorelli,Gianni Ciofani

Clinical cancer research : an official journal of the American Association for Cancer Research 29:2324-2335 PubMed36939530

2023

Expression and Therapeutic Targeting of TROP-2 in Treatment-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie M Sperger,Kyle T Helzer,Charlotte N Stahlfeld,Dawei Jiang,Anupama Singh,Katherine R Kaufmann,David J Niles,Erika Heninger,Nicholas R Rydzewski,Liguo Wang,Liewei Wang,Rendong Yang,Yanan Ren,Jonathan W Engle,Peng Huang,Christos E Kyriakopoulos,Susan F Slovin,Howard R Soule,Shuang G Zhao,Manish Kohli,Scott T Tagawa,Weibo Cai,Scott M Dehm,Joshua M Lang

Frontiers in medicine 9:904295 PubMed35899209

2022

Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Hofmann,Valentin Medyany,Jasmin Ezić,Ramin Lotfi,Beate Niesler,Ralph Röth,Daphne Engelhardt,Simon Laban,Patrick J Schuler,Thomas K Hoffmann,Cornelia Brunner,Edwin K Jackson,Marie-Nicole Theodoraki

Signal transduction and targeted therapy 7:46 PubMed35169126

2022

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Zhang,Qiuping He,Yaqin Wang,Guanqun Zhou,Yupei Chen,Linglong Tang,Yuan Zhang,Xiaohong Hong,Yanping Mao,Qingmei He,Xiaojing Yang,Na Liu,Jun Ma

Molecular oncology 15:2330-2344 PubMed33604999

2021

Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rory M Bade,Jennifer L Schehr,Hamid Emamekhoo,Benjamin K Gibbs,Tamara S Rodems,Matthew C Mannino,Joshua A Desotelle,Erika Heninger,Charlotte N Stahlfeld,Jamie M Sperger,Anupama Singh,Serena K Wolfe,David J Niles,Waddah Arafat,John A Steinharter,E Jason Abel,David J Beebe,Xiao X Wei,Rana R McKay,Toni K Choueri,Joshua M Lang

Lab on a chip 20:4420-4432 PubMed33103699

2020

Organotypic primary blood vessel models of clear cell renal cell carcinoma for single-patient clinical trials.

Applications

Unspecified application

Species

Unspecified reactive species

María Virumbrales-Muñoz,Jiong Chen,Jose Ayuso,Moonhee Lee,E Jason Abel,David J Beebe

Cell research 30:950-965 PubMed32901110

2020

Single-cell transcriptomic analysis defines the interplay between tumor cells, viral infection, and the microenvironment in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shanzhao Jin,Ruoyan Li,Ming-Yuan Chen,Chao Yu,Lin-Quan Tang,Yan-Min Liu,Jiang-Ping Li,Yi-Na Liu,Yi-Ling Luo,Yifan Zhao,Yu Zhang,Tian-Liang Xia,Shang-Xin Liu,Qi Liu,Guan-Nan Wang,Rui You,Jing-Yun Peng,Jiang Li,Feng Han,Jianwei Wang,Qiu-Yan Chen,Li Zhang,Hai-Qiang Mai,Benjamin E Gewurz,Bo Zhao,Lawrence S Young,Qian Zhong,Fan Bai,Mu-Sheng Zeng

Theranostics 10:11026-11048 PubMed33042268

2020

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kok Suen Cheng,Rongbin Pan,Huaping Pan,Binglin Li,Stephene Shadrack Meena,Huan Xing,Ying Jing Ng,Kaili Qin,Xuan Liao,Benson Kiprono Kosgei,Zhipeng Wang,Ray P S Han
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com