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Rabbit Recombinant Monoclonal Estrogen Receptor alpha antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 3 publications.


Images

Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (AB209288), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Anti-Estrogen Receptor alpha antibody [E115] (AB209288), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (AB209288), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (AB209288), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Tested
Dog
Not recommended
Not recommended
Predicted

Not recommended
Not recommended

Species
Mouse, Human, Rat, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min)

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min)

Species
Rat
Dilution info
-
Notes

This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min)

Species
Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/50 - 1/2500
Notes

-

Species
Mouse
Dilution info
1/50 - 1/2500
Notes

-

Species
Human
Dilution info
1/50 - 1/2500
Notes

-

Predicted
Predicted

Species
Dog
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

3 products for Alternative Version

Target data

Function

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity). Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861).

Alternative names

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Recommended products

Rabbit Recombinant Monoclonal Estrogen Receptor alpha antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
E115
Purification technique
Affinity purification Protein A
Specificity

Expression levels of ER alpha protein vary with sample type.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.

Biological function summary

ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.

Pathways

ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.

Associated diseases and disorders

ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288)

    Overlay histogram showing T47D cells stained with ab209288 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209288, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288)

    ab209288 staining Estrogen Receptor alpha in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209288 at 1/500 dilution (Pseudocolored in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288)

    Intracellular Flow Cytometry analysis of GH3 (rat pituitary epithelial) cells labeling Estrogen Receptor alpha with ab209288 at 1/50 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. Isotype control - Rabbit IgG monoclonal [EPR25A, PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478] (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-Estrogen Receptor alpha antibody [E115] (ab209288)

    Intracellular Flow Cytometry analysis of 4T1 (mouse mammary gland carcinoma epithelial cell) cells labeling Estrogen Receptor alpha with ab209288 at 1/50 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. Isotype control - Rabbit IgG monoclonal [EPR25A, PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478] (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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