AB105156

PE Anti-F4/80 antibody [CI:A3-1]

Name: PE Anti-F4/80 antibody [CI:A3-1] (ab105156) | Abcam
Brand: Abcam Limited
SKU: AB105156
Price: 525 USD
Availability: InStock

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(13 Publications)

Rat Monoclonal F4/80 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 13 publications. Immunogen corresponding to Cell preparation containing Adgre1 protein.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Flow Cytometry - PE Anti-F4/80 antibody [CI:A3-1] (AB105156)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-F4/80 antibody [CI:A3-1] (AB105156)

Flow cytometry staining of mouse J774 cells with ab105156.

Unconjugated

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker
660 APC

APC Anti-F4/80 antibody [CI:A3-1]
519 FITC

FITC Anti-F4/80 antibody [CI:A3-1]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CI:A3-1

Isotype

IgG2b

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61549

Specificity

ab105156 recognises the murine F4/80 antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>in 100 μl.</p><p><a href='/en-us/products/primary-antibodies/r-pe-rat-igg2b-kappa-monoclonal-klh-g2b-1-2-isotype-control-ab136585'>ab136585</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 5% Sucrose, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica : PubMed40551711

2025

Single-cell and bulk transcriptome analysis unveils a ligand-receptor-based signature for prognostication and reveals that TREM1 controls the malignant behaviors of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiemin Zhang,Qian Huang,Yingying Zheng,Jianqing Tang,Naling Kang,Yurui Liu,Dawu Zeng

Nature immunology 25:1820-1829 PubMed39112631

2024

Local delivery of cell surface-targeted immunocytokines programs systemic antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Luciano Santollani,Laura Maiorino,Yiming J Zhang,Joseph R Palmeri,Jordan A Stinson,Lauren R Duhamel,Kashif Qureshi,Jack R Suggs,Owen T Porth,William Pinney,Riyam Al Msari,Agnes A Walsh,K Dane Wittrup,Darrell J Irvine

Obesity facts 17:24-36 PubMed37820603

2023

Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqian Li,Xiao Dong,Wenqian He,Huibiao Quan,Kaining Chen,Chaoping Cen,Weiping Wei

NPJ Regenerative medicine 8:28 PubMed37270633

2023

Janus porous polylactic acid membranes with versatile metal-phenolic interface for biomimetic periodontal bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Zhang,Yi Chen,Tian Ding,Yandi Zhang,Daiwei Yang,Yajun Zhao,Jin Liu,Baojin Ma,Alberto Bianco,Shaohua Ge,Jianhua Li

Nature communications 14:1213 PubMed36869026

2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lai Yee Cheong,Baile Wang,Qin Wang,Leigang Jin,Kelvin H M Kwok,Xiaoping Wu,Lingling Shu,Huige Lin,Sookja Kim Chung,Kenneth K Y Cheng,Ruby L C Hoo,Aimin Xu

Mediators of inflammation 2022:1108569 PubMed36619207

2022

Prevents Ulcerative Colitis by Promoting M2 Macrophage Polarization through Blocking the TLR4/MAPK/NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Ping Zhao,Xufeng Ding,Fang Li,Jie Jiang,Hua Huang,Lijiang Ji

Cellular & molecular biology letters 26:30 PubMed34174813

2021

Macrophage polarization by MSC-derived CXCL12 determines tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Shabnam Babazadeh,Seyed Mahdi Nassiri,Vahid Siavashi,Mohadeseh Sahlabadi,Mostafa Hajinasrollah,Mohamad Zamani-Ahmadmahmudi

Cell death & disease 11:1016 PubMed33247124

2020

Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Aisong Guo,Yuanyuan Tu,Wendie Li,Lele Li,Wangrui Liu,Yuanyuan Ju,Yamei Zhou,Aimin Sang,Manhui Zhu

In vitro cellular & developmental biology. Animal 55:436-444 PubMed31119642

2019

A simple culture method for liver and intestinal tissue-resident macrophages from neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Shimizu,Naoya Sakuragi,Kiminori Nakamura,Toshio Taira,Tokiyoshi Ayabe,Akimasa Fukui

Nature communications 9:272 PubMed29348470

2018

Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat.

Applications

Unspecified application

Species

Unspecified reactive species

Huating Li,Guangyu Wu,Qichen Fang,Mingliang Zhang,Xiaoyan Hui,Bin Sheng,Liang Wu,Yuqian Bao,Peng Li,Aimin Xu,Weiping Jia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-F4/80 antibody [CI:A3-1]

Flow Cytometry - PE Anti-F4/80 antibody [CI:A3-1] (AB105156)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise