AB237253

PE Anti-Firefly Luciferase antibody [EPR17789]

RabMAb
Recombinant
What is this?

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Anti-Firefly Luciferase antibody [EPR17789] - PE conjugated (ab237253) is a rabbit recombinant monoclonal antibody detecting Firefly Luciferase in Flow Cytometry (Intra), Flow Cytometry. Suitable for Firefly.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Luciferin 4-monooxygenase, Luciferase

1 Images

Flow Cytometry (Intracellular) - PE Anti-Firefly Luciferase antibody [EPR17789] (AB237253)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Firefly Luciferase antibody [EPR17789] (AB237253)

HEK-293T cells transfected with GFP-tagged Firefly Luciferase stained with ab237253 (right). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS/ 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237253 (right)) or Rabbit IgG (monoclonal) Phycoerythrin Isotype control (ab209478 (left)) (1x10⁶ in 100μl at 1 : 50 dilution (10 μg/ml)) for 30 min at 22°C.

Acquisition of >5,000 events were collected using a 20 mW Blue laser (488 nm) and 585/42 bandpass filter.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Firefly Luciferase antibody [EPR17789]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Firefly Luciferase antibody [EPR17789]
HRP

HRP Anti-Firefly Luciferase antibody [EPR17789] - N-terminal
Unconjugated

Anti-Firefly Luciferase antibody [EPR17789] - N-terminal
Carrier free

Anti-Firefly Luciferase antibody [EPR17789] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17789

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Firefly

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Firefly": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
PE Anti-Firefly Luciferase antibody [EPR17789] (ab237253) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Firefly samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR17789 is also available pre-conjugated to a variety of labels for your convenience – Anti-Firefly Luciferase PE [EPR17789] (ab237253).

Other related products
We have a range of other formats of antibody clone [EPR17789] also available for your convenience: ab185923, Alexa Fluor® 488 - ab237251, Alexa Fluor® 647 - ab237252, PE - ab237253, Carrier free - ab238448

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.

Biological function summary

Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.

Pathways

Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.

Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces green light with a wavelength of 562 nm.

See full target information Luciferin 4-monooxygenase

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Firefly Luciferase antibody [EPR17789]

Flow Cytometry (Intracellular) - PE Anti-Firefly Luciferase antibody [EPR17789] (AB237253)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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