AB223948

PE Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal beta 2 Microglobulin antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

1 Images

Flow Cytometry (Intracellular) - PE Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB223948)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB223948)

Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab223948 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223948, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

660 APC

APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
HRP

HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4805

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab223948 will also recognize related targets GABARAPL1 and GABARAPL2: GABARAPL1: pBLAST 100% homology with immunogen. GABARAPL2: pBLAST 93% homolgy with immunogen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

Additional targets

GABARAPL1,GABARAPL2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113287 PubMed40894917

2025

Conserved LIR-specific interaction of Sigma-1 receptor and GABARAP.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Wilhelm Baeken,Maximilian Christ,Daniel Schmitt,Wencke Trein,Heike Nagel,Albrecht Martin Clement,Hagen Körschgen,Christian Behl

Journal of tissue engineering 15:20417314241306681 PubMed39712077

2024

Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects.

Applications

Unspecified application

Species

Unspecified reactive species

Keyu Luo,Yufei Jin,Baiyi Liu,Yingbo Wang,Yaoyao Liu,Si Qiu,Jianhua Zhao,Xiang Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

Flow Cytometry (Intracellular) - PE Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB223948)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (2)

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