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AB303588

PE Anti-GFP antibody [EPR14104]

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(1 Publication)

Rabbit Recombinant Monoclonal GFP antibody - conjugated to PE. Suitable for Flow Cyt (Intra).

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

1 Images
Flow Cytometry (Intracellular) - PE Anti-GFP antibody [EPR14104] (AB303588)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-GFP antibody [EPR14104] (AB303588)

Flow cytometry staining of 293T (human embryonic kidney) cells transfected with human TNFRSF9 labeling GFP stained with ab303588 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype control (left). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab303588) (1x 106 in 100 µl at 1 µg/ml (1/500)) for 30 min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter.

  • 660 APC

    APC Anti-GFP antibody [EPR14104]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GFP antibody [EPR14104] - GFP booster

  • Unconjugated

    Anti-GFP antibody [EPR14104]

  • Carrier free

    Anti-GFP antibody [EPR14104] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14104

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB235672

GFP Quantification Kit

Functional Studies - GFP Quantification Kit (AB235672)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB283597

APC Anti-GFP antibody [EPR14104]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - APC Anti-GFP antibody [EPR14104] (AB283597)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8530 PubMed39358385

2024

Interleukin-11 causes alveolar type 2 cell dysfunction and prevents alveolar regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Ng,Kevin Y Huang,Chee Jian Pua,Sivakumar Viswanathan,Wei-Wen Lim,Fathima F Kuthubudeen,Yu-Ning Liu,An An Hii,Benjamin L George,Anissa A Widjaja,Enrico Petretto,Stuart A Cook
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com