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AB326146

PE Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

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Rabbit Recombinant Monoclonal Glucose 6 Phosphate Dehydrogenase antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Glucose-6-phosphate 1-dehydrogenase, G6PD

1 Images
Flow Cytometry (Intracellular) - PE Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB326146)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB326146)

Flow cytometry scatter plots showing untreated human peripheral mononuclear cells (PBMCs) (top) and PBMCs activated with 5 µg/ml PHA for 18h (bottom), either stained with ab326146 (right) or isotype control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 µg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326146) (1x 106 in 100µl at 0.2 µg/ml (1/2500)) for 30min on ice. Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

  • Carrier free

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

  • Unconjugated

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20668

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-Δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
See full target information Glucose-6-phosphate 1-dehydrogenase
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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