PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (AB326128)

Flow cytometry overlay histogram showing wild-type HeLa (green line) and GYS1 knockout HeLa stained with ab326128 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab326128) (1x 106 in 100µl at 0.0016 µg/ml (1/312500)) for 30min at 22°C.Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (black line) in HeLa WT cells (black line) and HeLa-GYS1 KO cells (grey line), used at the same concentration and conditions as the primary antibody. Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.This antibody gave a positive signal in wild-type HeLa fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.