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AB314286

PE Anti-GPCR LGR6 antibody [EPR6874]

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Rabbit Recombinant Monoclonal GPCR LGR6 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

UNQ6427/PRO21331, VTS20631, LGR6, Leucine-rich repeat-containing G-protein coupled receptor 6

1 Images
Flow Cytometry (Intracellular) - PE Anti-GPCR LGR6 antibody [EPR6874] (AB314286)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-GPCR LGR6 antibody [EPR6874] (AB314286)

Flow cytometry overlay histogram showing HeLa cells stained with ab314286 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314286) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6874

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/12500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPCR LGR6 also known as leucine-rich repeat-containing G protein-coupled receptor 6 is a transmembrane receptor that belongs to the large GPCR family. It has a molecular weight of approximately 98 kDa. LGR6 expresses in various tissues including skin bone and certain regions of the brain. The receptor binds specific ligands which initiates signal transduction pathways that mediate various cellular responses. This function places LGR6 as an important component in multiple physiological processes.
Biological function summary

LGR6 plays an important role in signaling pathways involved in stem cell maintenance and tissue regeneration. It functions together with other proteins as part of a larger receptor complex. In the skin LGR6 marks a population of stem cells involved in hair follicle cycling and epidermal renewal. Its activity influences the cellular communication required for tissue homeostasis and repair. This receptor helps in determining cellular fate and proliferation in various tissues.

Pathways

LGR6 participates in the Wnt signaling pathway which is important for cell differentiation growth and migration. LGR6 interacts with proteins such as R-spondins enhancing Wnt signaling. This connection highlights LGR6's role in modulating pathways essential for embryonic development and tissue patterning. The interaction with Wnt and related proteins positions LGR6 as a modulator of pathways that govern stem cell behavior.

Abnormalities in LGR6 function have associations with skin disorders and cancers. Mutations or dysregulation of LGR6 can lead to impaired wound healing and contribute to pathological conditions. In cancer LGR6 has connections with proteins that affect tumor growth and metastasis. Studies suggest involvement of LGR6 in pathways related to squamous cell carcinoma further linking it with proteins involved in cancer progression therefore underlining its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
See full target information LGR6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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