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Rabbit Recombinant Monoclonal GRP94 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Flow Cytometry (Intracellular) - PE Anti-GRP94 antibody [EPR22847-50] (AB322454), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Anti-GRP94 antibody [EPR22847-50] (AB322454), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Anti-GRP94 antibody [EPR22847-50] (AB322454), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Anti-GRP94 antibody [EPR22847-50] (AB322454), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Species

Mouse

Dilution info

1/250

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Associated Products

Select an associated product type

1 product for Isotype control

Target data

Function

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).

Alternative names

GRP94, HSPC4, TRA1, GRP94, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94

Rabbit Recombinant Monoclonal GRP94 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22847-50

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.

Biological function summary

GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.

Pathways

GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.

Associated diseases and disorders

GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - PE Anti-GRP94 antibody [EPR22847-50] (ab322454), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-GRP94 antibody [EPR22847-50] (ab322454)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling GRP94 with ab322454 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Flow Cytometry (Intracellular) - PE Anti-GRP94 antibody [EPR22847-50] (ab322454), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-GRP94 antibody [EPR22847-50] (ab322454)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling GRP94 with ab322454 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • Immunocytochemistry/ Immunofluorescence - PE Anti-GRP94 antibody [EPR22847-50] (ab322454), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Anti-GRP94 antibody [EPR22847-50] (ab322454)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling GRP94 with ab322454 at 1/250 (10.0 ug/ml) dilution.

    Confocal image showing cytoplasmic staining in NIH/3T3 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - PE Anti-GRP94 antibody [EPR22847-50] (ab322454), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Anti-GRP94 antibody [EPR22847-50] (ab322454)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling GRP94 with ab322454 at 1/250 (10.0 ug/ml) dilution.

    Confocal image showing cytoplasmic staining in HeLa cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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