AB83214

PE Anti-Heme Oxygenase 1 antibody [HO-1-2]

Name: PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab83214) | Abcam
Brand: Abcam Limited
SKU: AB83214

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(6 Publications)

Mouse Monoclonal Heme Oxygenase 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Hmox1.

View Alternative Names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

1 Images

Flow Cytometry (Intracellular) - PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB83214)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB83214)

10⁶ Jurkat cells stained using ab83214 at a concentration of 10 μg/mL.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HO-1-2

Isotype

IgG2b

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Native Full Length Protein corresponding to Rat Hmox1.

P06762

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Reactivity data

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Product details

This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:3983-4000 PubMed39309491

2024

Heme oxygenase 1-mediated ferroptosis in Kupffer cells initiates liver injury during heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Li,Riqing Wei,Chenxin Liu,Keying Zhang,Sixiao He,Zhifeng Liu,Junhao Huang,Youyong Tang,Qiyuan An,Ligen Lin,Lishe Gan,Liying Zhao,Xiaoming Zou,Fudi Wang,Yuan Ping,Qiang Ma

Cancer immunology, immunotherapy : CII 72:2233-2244 PubMed36869896

2023

M2 tumor-associated macrophages resist to oxidative stress through heme oxygenase-1 in the colorectal cancer tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Misato Ito,Kosaku Mimura,Shotaro Nakajima,Hirokazu Okayama,Katsuharu Saito,Takahiro Nakajima,Tomohiro Kikuchi,Hisashi Onozawa,Shotaro Fujita,Wataru Sakamoto,Motonobu Saito,Tomoyuki Momma,Zenichiro Saze,Koji Kono

Journal of immunology (Baltimore, Md. : 1950) 206:1493-1504 PubMed33627377

2021

Conserved Epigenetic Programming and Enhanced Heme Metabolism Drive Memory B Cell Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Madeline J Price,Christopher D Scharer,Anna K Kania,Troy D Randall,Jeremy M Boss

Cell death and differentiation 24:1224-1238 PubMed28524859

2017

Mesenchymal stem cells sense mitochondria released from damaged cells as danger signals to activate their rescue properties.

Applications

Flow Cyt

Species

Human

Meriem Mahrouf-Yorgov,Lionel Augeul,Claire Crola Da Silva,Maud Jourdan,Muriel Rigolet,Sylvie Manin,René Ferrera,Michel Ovize,Adeline Henry,Aurélie Guguin,Jean-Paul Meningaud,Jean-Luc Dubois-Randé,Roberto Motterlini,Roberta Foresti,Anne-Marie Rodriguez

Scientific reports 7:755 PubMed28389660

2017

Characteristics of the heme catabolic pathway in mild unconjugated hyperbilirubinemia and their associations with inflammation and disease prevention.

Applications

Flow Cyt

Species

Human

Christine Mölzer,Marlies Wallner,Carina Kern,Anela Tosevska,René Zadnikar,Daniel Doberer,Rodrig Marculescu,Karl-Heinz Wagner

Endocrinology 153:4367-79 PubMed22733969

2012

Immunoregulatory natural killer cells suppress autoimmunity by down-regulating antigen-specific CD8+ T cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Margret Ehlers,Claudia Papewalis,Wiebke Stenzel,Benedikt Jacobs,Klaus L Meyer,René Deenen,Holger S Willenberg,Sven Schinner,Annette Thiel,Werner A Scherbaum,Evelyn Ullrich,Laurence Zitvogel,Matthias Schott

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-Heme Oxygenase 1 antibody [HO-1-2]

Flow Cytometry (Intracellular) - PE Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB83214)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise