PE Anti-Histone H2A.X antibody [EPR22820-23] - ChIP Grade

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Histone H2A.X antibody [EPR22820-23] - ChIP Grade (AB314253)

Flow cytometry overlay histogram showing HeLa cells stained with ab314253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314253) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.