AB209942

PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

Name: PE conjugated Anti-Iba1 antibody [EPR6136(2)] (ab209942) | Abcam
Brand: Abcam Limited
SKU: AB209942
Price: 560 USD
Availability: InStock
Rating: 5 (4 reviews)

RabMAb
Recombinant
What is this?

(4 Reviews)

(2 Publications)

Anti-Iba1 antibody [EPR6136(2)] - PE conjugated (ab209942) is a rabbit recombinant monoclonal antibody detecting Iba1 in Flow Cytometry (Intra). Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1

1 Images

Flow Cytometry (Intracellular) - PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB209942)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB209942)

Overlay histogram showing K562 cells stained with ab209942 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209942, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Carrier free

Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free
Unconjugated

Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6136(2)

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
PE Anti-Iba1 antibody [EPR6136(2)] (ab209942) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone EPR6136(2) is also available pre-conjugated to a variety of labels for your convenience – Anti-Iba1 PE [EPR6136(2)] (ab209942).

Other related products
We have a range of other formats of antibody clone [EPR6136(2)] also available for your convenience: ab178680, Alexa Fluor® 488 - ab195031, Alexa Fluor® 647 - ab195032, PE - ab209942, Alexa Fluor® 568 - ab221003, Carrier free - ab221933

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.

Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

See full target information AIF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 45:7827-7841 PubMed37886937

2023

T-Lymphocytes Activated by Dendritic Cells Loaded by Tumor-Derived Vesicles Decrease Viability of Melanoma Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Yurevich Filin,Yuriy Pavlovich Mayasin,Chulpan Bulatovna Kharisova,Anna Valerevna Gorodilova,Daria Sergeevna Chulpanova,Kristina Viktorovna Kitaeva,Albert Anatolyevich Rizvanov,Valeria Vladimirovna Solovyeva

Cells 11: PubMed35406690

2022

Single-Cell Molecular Characterization to Partition the Human Glioblastoma Tumor Microenvironment Genetic Background.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Lessi,Sara Franceschi,Mariangela Morelli,Michele Menicagli,Francesco Pasqualetti,Orazio Santonocito,Carlo Gambacciani,Francesco Pieri,Filippo Aquila,Paolo Aretini,Chiara Maria Mazzanti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker

Flow Cytometry (Intracellular) - PE Anti-Iba1 antibody [EPR6136(2)] - Microglia marker (AB209942)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise