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Rabbit Recombinant Monoclonal ICOS antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.


Images

Flow Cytometry - PE Anti-ICOS antibody [EPR22181] (AB245909), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Anti-ICOS antibody [EPR22181] (AB245909), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed:9930702). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed:33033255). Acts also as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed:27135603). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (By similarity). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed:30523347). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ICOS antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22181

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - PE Anti-ICOS antibody [EPR22181] (ab245909), expandable thumbnail

    Flow Cytometry - PE Anti-ICOS antibody [EPR22181] (ab245909)

    Flow cytometric analysis of 293T transfected with GFP-tagged ICOS overexpression vector using PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (Left panel) and ab245909 at 1/500 dilution (Right panel).

    Blocking: 10% Goat serum.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-ICOS antibody [EPR22181] (ab245909), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Anti-ICOS antibody [EPR22181] (ab245909)

    Immunocytochemistry/ Immunofluorescence analysis of 4% PFA-fixed, 0.1% Triton X-100-permeabilized 293T (human embryonic kidney epithelial cell) cells using Alexa Fluor® 647 Anti-ICOS antibody [EPR22181] ab245908 at 1/200 dilution (2.5 μg/ml) (red). Counterstain: DAPI (blue).

    Confocal image showing increased cytoplasmic staining in 293T cells transfected with ICOS with GFP tag.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com