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AB95673

PE Anti-IFNGR1 antibody [2E2]

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(4 Publications)

Armenian hamster Monoclonal IFNGR1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Ifngr1.

View Alternative Names

CD119, Ifngr, Ifngr1, Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha

1 Images
Flow Cytometry - PE Anti-IFNGR1 antibody [2E2] (AB95673)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-IFNGR1 antibody [2E2] (AB95673)

Flow cytometry staining of BALB/c splenocytes with : (1) ab95673 at 1 µg and (2) Phycoerythrin conjugated Armenian Hamster IgG Isotype Control at 1 µg. Total viable cells were used for analysis.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

2E2

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Ifngr1.

P15261

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>Cell number should be determined empirically but can range from 10<sup>5</sup> to 10<sup>8</sup> cells/test (based on a test volume of 100 μl).</p><p>ab101026 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFNGR1 protein also known as Interferon Gamma Receptor 1 is an integral component of the cell surface receptor for interferon-gamma (IFN-γ). It possesses a mass of approximately 53 kDa. IFNGR1 is expressed in various cell types including immune cells such as T cells and macrophages and is also present in other cell lines like HEK 293. This receptor binds to its specific ligand IFN-γ initiating a series of intracellular events that are essential for immune response modulation.
Biological function summary

IFNGR1 functions as part of the interferon-gamma receptor complex working alongside the IFNGR2 subunit. This interaction with IFNGR2 is important for the receptor to properly transmit signals inside the cell. The binding of interferon-gamma to this receptor complex activates the JAK-STAT signaling pathway promoting the transcription of genes that enhance the antimicrobial activity of immune cells and regulate cellular immunity.

Pathways

The IFNGR1 protein plays an important role in the JAK-STAT signaling pathway which is critical for mediating responses to interferon-gamma. Upon activation by IFN-γ IFNGR1 recruits JAK kinases leading to the phosphorylation of STAT1 a significant transcription factor. STAT1 then dimerizes and translocates to the nucleus where it induces the expression of genes involved in immune defense and inflammation. This process is vital for the body's ability to handle infections and other immunological challenges.

IFNGR1 is associated with susceptibility to infections and certain immune-related disorders. Mutations or deficiencies in IFNGR1 can lead to Mendelian Susceptibility to Mycobacterial Disease (MSMD) where individuals show increased vulnerability to mycobacterial infections. Additionally improper signaling through IFNGR1 is linked to chronic granulomatous disease which can involve defective functioning of the NADPH oxidase complex. Understanding IFNGR1's function and interactions is important for exploring new therapeutic strategies for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed : 20926559, PubMed : 27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed : 2137461, PubMed : 2530216, PubMed : 2530582, PubMed : 2531896, PubMed : 2532365). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed : 19889125). STAT3 can also be activated in a similar manner although activation seems weaker (PubMed : 15284232). IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (PubMed : 15522878).
See full target information Ifngr1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 22:550 PubMed34093771

2021

Formosanin C promotes the curative efficacy of ultrasound-guided radiofrequency ablation in a mouse model of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Chen,Jing Li,Qianqian Cui,Fuyuan Li,Gaiying Zhang

Cell 175:1336-1351.e17 PubMed30318148

2018

Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Xu,Jia Xu,Jiacheng Wu,Ye Hu,Yanmei Han,Yan Gu,Kai Zhao,Qian Zhang,Xingguang Liu,Juan Liu,Bing Liu,Xuetao Cao

Genes & development 28:2597-612 PubMed25395663

2014

Inflammatory signaling regulates embryonic hematopoietic stem and progenitor cell production.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Virginie Esain,Li Teng,Jian Xu,Wanda Kwan,Isaura M Frost,Amanda D Yzaguirre,Xiongwei Cai,Mauricio Cortes,Marijke W Maijenburg,Joanna Tober,Elaine Dzierzak,Stuart H Orkin,Kai Tan,Trista E North,Nancy A Speck

European journal of immunology 36:73-81 PubMed16342233

2005

Complex regulation of CCR9 at multiple discrete stages of T cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Marc-André Wurbel,Bernard Malissen,James J Campbell
View all publications
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