AB232924

PE Anti-Ikaros antibody [4E9]

Name: Anti-Ikaros PE antibody [4E9] (ab232924) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB232924
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Ikaros antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IKZF1.

View Alternative Names

IK1, IKAROS, LYF1, ZNFN1A1, IKZF1, DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1

3 Images

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Intracellular staining of Ikaros in human peripheral blood leukocytes with ab232924. Gated on leukocytes.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using ab232924 (10 ul reagent/100ul of peripheral whole blood).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using ab232924 (10ul reagent/100ul of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4E9

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human IKZF1. The exact immunogen used to generate this antibody is proprietary information.

Q13422

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ikaros also known as IKZF1 is a transcription factor. You also find it being referred to as the Ikaros protein or Ikaros code. It has a molecular mass of about 58-60 kDa. Ikaros expresses mostly in hematopoietic cells including precursors from which various blood cell types derive. It plays a significant role in regulating gene expression and cellular differentiation modulating the chromatin structure to control access to DNA.

Biological function summary

Ikaros protein functions in the regulation of lymphoid cell development. It is a part of a chromatin-remodeling complex influencing chromatin accessibility. Through its zinc finger domains it binds to specific DNA sequences controlling genes necessary for lymphocyte differentiation and proliferation. Ikaros influences various cell types within the immune system especially affecting T and B cell development and also regulates cytokine receptor genes necessary for proper immune function.

Pathways

The Ikaros protein is critical to lymphocyte signaling pathways particularly the JAK/STAT and NF-κB pathways. In these pathways Ikaros interacts with other transcription factors like GATA3 and STAT5 integrating signals that are important for immune cell function and development. This integration allows a coordinated response to extracellular signals ensuring effective immune responses or tolerance.

Ikaros is associated with leukemia and lymphomas. Alterations or mutations in ikaros gene can lead to misregulation of its target genes contributing to the development of these diseases by disturbing normal hematopoietic processes. Moreover its interaction with TAL1 protein is significant in certain leukemias where disturbances in the balance of their activities can lead to malignancies. Understanding Ikaros’s role provides insights for therapeutic approaches in these blood disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcription regulator of hematopoietic cell differentiation (PubMed : 17934067). Binds gamma-satellite DNA (PubMed : 17135265, PubMed : 19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed : 17135265, PubMed : 17934067).

See full target information IKZF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Folia histochemica et cytobiologica 63:11-27 PubMed40152123

2025

Inhibition of STAT1 alleviates oxidative damage in retinal pigment epithelial cells and exhibits neuroprotective effects in autoimmune optic neuritis by upregulating IFI30 lysosomal thiol reductase.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Ma,Jiajia Yuan

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-Ikaros antibody [4E9]

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Flow Cytometry (Intracellular) - PE Anti-Ikaros antibody [4E9] (AB232924)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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