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AB282176

PE Anti-IL-33 antibody [EPR17831]

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(1 Publication)

Rabbit Recombinant Monoclonal IL-33 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Interleukin-33, IL-33, Il33

1 Images
Flow Cytometry (Intracellular) - PE Anti-IL-33 antibody [EPR17831] (AB282176)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-IL-33 antibody [EPR17831] (AB282176)

Flow cytometry overlay histogram showing RAW 264.7 cells treated with 50nM PMA and 5μg/ml LPS for 24h (green line) and RAW 264.7 cells stained with ab282176 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml anti CD16/CD32 and 10 % normal goat serum followed by the antibody (ab282176) (1x 106 in 100μl at 0.008μg/ml (1/2500) for 30 min at 22°C.

Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody (treated RAW 264.7 cells - black line, untreated RAW 264.7 cells - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

  • Carrier free

    Anti-IL-33 antibody [EPR17831] - BSA and Azide free

  • Unconjugated

    Anti-IL-33 antibody [EPR17831]

  • 660 APC

    APC Anti-IL-33 antibody [EPR17831]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-IL-33 antibody [EPR17831]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17831

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB223865

Human IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-33 ELISA Kit (AB223865)

  • 0
  • 0
Proteins & Peptides

AB281811

Recombinant Human IL-33 protein (Active)

Premium Bioactive

Functional Studies - Recombinant Human IL-33 protein (Active) (AB281811)

  • 0
  • 0
Assay Kits

AB213475

Mouse IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-33 ELISA Kit (AB213475)

  • 5
  • 2
Primary Antibodies

AB309480

Anti-TSLP antibody [EPR25904-38] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-TSLP antibody [EPR25904-38] - BSA and Azide free (AB309480)

  • 0
  • 0
Primary Antibodies

AB25877

Anti-ST2 antibody - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST2 antibody - BSA free (AB25877)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed : 29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (By similarity). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (By similarity). Acts as an enhancer of polarization of alternatively activated macrophages (By similarity). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed : 34644537).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (By similarity). This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).
See full target information Il33
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:463 PubMed35568711

2022

m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Xian Lin,Ning-Zi Lian,You-Xin Gao,Qiao-Ling Zheng,Ying-Hong Yang,Yu-Bin Ma,Zhi-Song Xiu,Qing-Zhu Qiu,Hua-Gen Wang,Chao-Hui Zheng,Ping Li,Jian-Wei Xie,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Jia-Bin Wang,Chang-Ming Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com