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AB326522

PE Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)]

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Rat Monoclonal Integrin alpha 5 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD49e, Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, Itga5

2 Images
Flow Cytometry - PE Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)] (AB326522)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)] (AB326522)

Separation of murine CD49e positive SSC low cells (red-filled) from CD49e negative SSC low cells (black-dashed) in flow cytometry analysis (surface staining) of murine bone marrow cells stained using ab326522 (concentration in sample 0.19 μg/ml).

Flow Cytometry - PE Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)] (AB326522)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)] (AB326522)

Flow cytometry surface staining pattern of murine bone marrow cells stained using ab326522 (concentration in sample 0.19 μg/ml).

  • Unconjugated

    Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)]

  • 519 FITC

    FITC Anti-Integrin alpha-5 antibody [5H10-27 (MFR5)]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD49e antibody [5H10-27 (MFR5)]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

5H10-27 (MFR5)

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-5/beta-1 (ITGA5 : ITGB1) is a receptor for fibronectin and fibrinogen (PubMed : 36812915). It recognizes the sequence R-G-D in its ligands. ITGA5 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5 : ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5 : ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1 (By similarity). ITGA5 : ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5 : ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (By similarity).
See full target information Integrin alpha-5
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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