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AB95703

PE Anti-Integrin alpha 6 antibody [GoH3]

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(12 Publications)

Rat Monoclonal Integrin alpha 6 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing Itga6 protein.

View Alternative Names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6

2 Images
Flow Cytometry - PE Anti-Integrin alpha 6 antibody [GoH3] (AB95703)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Integrin alpha 6 antibody [GoH3] (AB95703)

Flow Cytometry analysis of normal human peripheral blood cells stained with Rat IgG2a kappa Isotype Control Phycoerythrin (blue histogram) or Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) (purple histogram). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry - PE Anti-Integrin alpha 6 antibody [GoH3] (AB95703)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-Integrin alpha 6 antibody [GoH3] (AB95703)

Flow cytometry staining of normal Human peripheral blood cells with 0.06 μg of Rat IgG2a Isotype Control PE (open histogram) or 0.06 μg of ab95703 (filled histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

GoH3

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itga6 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61739

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.06-0.125 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>Staining the cell sample in a final volume of 100 μl is recommended.</p><p><a href='/en-us/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-pe-isotype-control-ab134674'>ab134674</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.
Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (PubMed : 8081870). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (PubMed : 10634791). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (PubMed : 8673141). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (By similarity). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (By similarity).
See full target information Itga6
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7470 PubMed36463236

2022

Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Gerdes,Sue Mei Lim,Adam D Ewing,Michael R Larcombe,Dorothy Chan,Francisco J Sanchez-Luque,Lucinda Walker,Alexander L Carleton,Cini James,Anja S Knaupp,Patricia E Carreira,Christian M Nefzger,Ryan Lister,Sandra R Richardson,Jose M Polo,Geoffrey J Faulkner

Journal of biophotonics 15:e202200217 PubMed36054075

2022

A transient photoactivation of epidermal stem cells by femtosecond laser promotes skin wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Bijin Hu,Xiaohui Zhao,Yiting Lu,Yujie Zhu,Hao He

Stem cell research & therapy 10:175 PubMed31196164

2019

Elevated level of mitochondrial reactive oxygen species via fatty acid β-oxidation in cancer stem cells promotes cancer metastasis by inducing epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Caihua Wang,Liming Shao,Chi Pan,Jun Ye,Zonghui Ding,Jia Wu,Qin Du,Yuezhong Ren,Chunpeng Zhu

EMBO reports 20:e47789 PubMed31267708

2019

Dynamic expression of tRNA-derived small RNAs define cellular states.

Applications

Unspecified application

Species

Unspecified reactive species

Srikar Krishna,Daniel Gr Yim,Vairavan Lakshmanan,Varsha Tirumalai,Judice Ly Koh,Jung Eun Park,Jit Kong Cheong,Joo Leng Low,Michelle Js Lim,Siu Kwan Sze,Padubidri Shivaprasad,Akash Gulyani,Srikala Raghavan,Dasaradhi Palakodeti,Ramanuj DasGupta

Cancer management and research 11:2203-2214 PubMed31114314

2019

Increased expression of TMED2 is an unfavorable prognostic factor in patients with breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Lin,Jian Liu,Shu Fang Hu,Xun Hu

International journal of nanomedicine 13:2943-2954 PubMed29849458

2018

Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ronak Shabani,Mohsen Ashjari,Khadijeh Ashtari,Fariborz Izadyar,Babak Behnam,Samideh Khoei,Mohamad Asghari-Jafarabadi,Morteza Koruji

International journal of molecular sciences 17: PubMed27898044

2016

DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization.

Applications

Unspecified application

Species

Unspecified reactive species

Hengguang Zhao,Sandra Rieger,Koichiro Abe,Martin Hewison,Thomas S Lisse

Andrologia 48:584-94 PubMed26428408

2015

In vitro toxicity assay of cisplatin on mouse acute lymphoblastic leukaemia and spermatogonial stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

R Shabani,K Ashtari,B Behnam,F Izadyar,H Asgari,M Asghari Jafarabadi,M Ashjari,E Asadi,M Koruji

Antimicrobial agents and chemotherapy 59:7290-8 PubMed26369967

2015

Griffithsin and Carrageenan Combination To Target Herpes Simplex Virus 2 and Human Papillomavirus.

Applications

Unspecified application

Species

Unspecified reactive species

Keith Levendosky,Olga Mizenina,Elena Martinelli,Ninochka Jean-Pierre,Larisa Kizima,Aixa Rodriguez,Kyle Kleinbeck,Thierry Bonnaire,Melissa Robbiani,Thomas M Zydowsky,Barry R O'Keefe,José A Fernández-Romero

PloS one 8:e61828 PubMed23613946

2013

Co-culture of neural crest stem cells (NCSC) and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death.

Applications

ICC/IF

Species

Mouse

Anongnad Ngamjariyawat,Kyril Turpaev,Svitlana Vasylovska,Elena N Kozlova,Nils Welsh
View all publications
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