JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB211845

PE Anti-Integrin alpha V antibody [EPR16800]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Integrin alpha V antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

1 Images
Flow Cytometry (Intracellular) - PE Anti-Integrin alpha V antibody [EPR16800] (AB211845)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Integrin alpha V antibody [EPR16800] (AB211845)

Overlay histogram showing A549 cells stained with ab211845 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211845, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Unconjugated

    Anti-Integrin alpha V antibody [EPR16800]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Integrin alpha V antibody [EPR16800]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800]

  • HRP

    HRP Anti-Integrin alpha V antibody [EPR16800]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Integrin alpha V antibody [EPR16800]

  • Carrier free

    Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16800

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

You may be interested in:

AB235650

Human CD51 ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.
Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
See full target information ITGAV
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB11470

Anti-Integrin alpha V antibody [P2W7]

primary-antibodies

integrin-alpha-v-antibody-p2w7-ab11470

0

(0 reviews)

Alternative Product
Primary Antibodies

AB16821

Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free

primary-antibodies

integrin-alpha-v-antibody-272-17e6-bsa-and-azide-free-ab16821

5

(4 reviews)

Alternative Product
Primary Antibodies

AB208012

Anti-Integrin alpha V antibody [EPR19669]

primary-antibodies

integrin-alpha-v-antibody-epr19669-ab208012

0

(0 reviews)

Alternative Product
Primary Antibodies

AB117611

Anti-Integrin alpha V antibody

primary-antibodies

integrin-alpha-v-antibody-ab117611

3

(1 reviews)

Alternative Product
Primary Antibodies

AB63490

Anti-Integrin alpha V antibody [RMV-7]

primary-antibodies

integrin-alpha-v-antibody-rmv-7-ab63490

0

(0 reviews)

Alternative Product
Primary Antibodies

AB124968

Anti-Integrin alpha V antibody [EPR5583]

primary-antibodies

integrin-alpha-v-antibody-epr5583-ab124968

4

(2 reviews)

Alternative Version
Primary Antibodies

AB275350

Alexa Fluor® 488 Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

alexa-fluor-488-integrin-alpha-v-antibody-epr16800-ab275350

0

(0 reviews)

Alternative Version
Primary Antibodies

AB207286

Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

alexa-fluor-594-integrin-alpha-v-antibody-epr16800-ab207286

0

(0 reviews)

Alternative Version
Primary Antibodies

AB205474

HRP Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

hrp-integrin-alpha-v-antibody-epr16800-ab205474

0

(0 reviews)

Alternative Version
Primary Antibodies

AB204684

Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

alexa-fluor-647-integrin-alpha-v-antibody-epr16800-ab204684

0

(0 reviews)

Alternative Version
Primary Antibodies

AB222222

Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free

primary-antibodies

integrin-alpha-v-antibody-epr16800-low-endotoxin-azide-free-ab222222

0

(0 reviews)

Alternative Version
Primary Antibodies

AB275351

Alexa Fluor® 555 Anti-Integrin alpha V antibody [EPR16800]

primary-antibodies

alexa-fluor-555-integrin-alpha-v-antibody-epr16800-ab275351

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com