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AB216310

PE Anti-Integrin beta 4 antibody [346-11A]

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(1 Publication)

Rat Monoclonal Integrin beta 4 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD104, Integrin beta-4, Itgb4

1 Images
Flow Cytometry - PE Anti-Integrin beta 4 antibody [346-11A] (AB216310)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Integrin beta 4 antibody [346-11A] (AB216310)

Flow cytometric analysis of mouse lung carcinoma cell line labeling Integrin beta 4 with ab216310 at 0.25 μg for 106 cells (filled histogram). A rat IgG2a, κ PE conjugate was used as isotype control (open histogram).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

346-11A

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>(in 100 μl volume).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab216310 was conjugated with Phycoerythrin under optimal conditions. The solution is free of unconjugated Phycoerythrin and unconjugated antibody.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.
Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
See full target information Integrin beta-4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 123:682-99 PubMed23348745

2013

β4 Integrin signaling induces expansion of prostate tumor progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiaki Yoshioka,Javier Otero,Yu Chen,Young-Mi Kim,Jason A Koutcher,Jaya Satagopan,Victor Reuter,Brett Carver,Elisa de Stanchina,Katsuhiko Enomoto,Norman M Greenberg,Peter T Scardino,Howard I Scher,Charles L Sawyers,Filippo G Giancotti
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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