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AB306063

PE Anti-JAK3 antibody [EP909Y]

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Rabbit Recombinant Monoclonal JAK3 antibody - conjugated to PE.

View Alternative Names

Tyrosine-protein kinase JAK3, Janus kinase 3, Leukocyte janus kinase, JAK-3, L-JAK, JAK3

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP909Y

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JAK3 also known as Janus kinase 3 is a protein with a molecular mass of approximately 125 kDa. It belongs to the Janus kinase family and plays an important role in the signal transduction pathways. JAK3 is expressed mainly in hematopoietic cells which include white blood cells and related precursor cells. Its primary function involves mediating signaling by associating with the common gamma chain used by cytokine receptors.
Biological function summary

JAK3 is instrumental in the signaling mechanism of the immune system. It participates in the activation of various interleukins specifically those that signal through the common gamma chain receptor complex. JAK3's role is essential in the proper functioning and development of immune cells such as T cells and natural killer cells. Within this framework JAK3 pairs with cytokine receptors transmitting signals that regulate both proliferation and differentiation of these immune cells.

Pathways

JAK3 integrates into JAK-STAT signaling pathways. This protein works alongside STAT proteins including STAT5 to facilitate the transcription of genes involved in cell survival and proliferation. It also interacts with JAK1 another member of the Janus kinase family to form functional complexes. These interactions are pivotal in maintaining normal immune responses and ensuring that signaling pathways are activated appropriately in response to extracellular cues.

Abnormal JAK3 function can lead to immune-related conditions. Severe combined immunodeficiency (SCID) is one such disorder arising from JAK3 mutations that impair immune cell function. Additionally JAK3 dysregulation links to malignancies such as T-cell acute lymphoblastic leukemia. Relating to these disorders JAK3 affects proteins like IL-7R and gamma chains which are important for cytokine signaling contributing to pathologies when malfunctioning.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
See full target information JAK3
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Product promise

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