AB131602

PE Anti-Kappa light chain antibody [TB28-2]

Name: PE Anti-Kappa light chain antibody [TB28-2] (ab131602) | Abcam
Brand: Abcam Limited
SKU: AB131602
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal IGKC antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human IGKC.

View Alternative Names

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

1 Images

Flow Cytometry - PE Anti-Kappa light chain antibody [TB28-2] (AB131602)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Kappa light chain antibody [TB28-2] (AB131602)

Analysis by flow cytometry of ab131602 staining Kappa light chains in human peripheral blood cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TB28-2

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human IGKC.

P01834

Specificity

ab131602 recognizes Human Ig kappa light chains of both secreted and cell surface immunoglobulin. It also detects free kappa light chains.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 12:26 PubMed35255981

2022

Generation of immunodeficient pig with hereditary tyrosinemia type 1 and their preliminary application for humanized liver.

Applications

Unspecified application

Species

Unspecified reactive species

Jilong Ren,Dawei Yu,Jing Wang,Kai Xu,Yanan Xu,Renren Sun,Peipei An,Chongyang Li,Guihai Feng,Ying Zhang,Xiangpeng Dai,Hongye Zhao,Zhengzhu Wang,Zhiqiang Han,Haibo Zhu,Yuchun Ding,Xiaoyan You,Xueqin Liu,Meng Wu,Lin Luo,Ziyi Li,Yong-Guang Yang,Zheng Hu,Hong-Jiang Wei,Liangpeng Ge,Tang Hai,Wei Li

PloS one 11:e0165385 PubMed27788210

2016

Nuclear COMMD1 Is Associated with Cisplatin Sensitivity in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Fedoseienko,Hylke W Wieringa,G Bea A Wisman,Evelien Duiker,Anna K L Reyners,Marten H Hofker,Ate G J van der Zee,Bart van de Sluis,Marcel A T M van Vugt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-Kappa light chain antibody [TB28-2]

Flow Cytometry - PE Anti-Kappa light chain antibody [TB28-2] (AB131602)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise