AB270650

PE Anti-Ki67 antibody [Ki-67]

Name: Anti-Ki67 PE antibody [Ki-67] (ab270650) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB270650
Price: 530 USD
Availability: InStock

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(11 Publications)

Mouse Monoclonal Ki67 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Cell preparation containing MKI67 protein.

View Alternative Names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

1 Images

Flow Cytometry (Intracellular) - PE Anti-Ki67 antibody [Ki-67] (AB270650)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Ki67 antibody [Ki-67] (AB270650)

Intracellular Flow Cytometry analysis ofhuman peripheralblood mononuclear cells stimulated with PHA labeling Ki67using ab270650.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Ki-67

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing MKI67 protein. The exact immunogen used to generate this antibody is proprietary information.

P46013

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed : 27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed : 27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed : 10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed : 24867636). This supplementary information is collated from multiple sources and compiled automatically.

See full target information MKI67

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:157 PubMed38733530

2024

circPDK1 competitively binds miR-4731-5p to mediate GIGYF1 expression and increase paclitaxel sensitivity in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

YunYin Feng,TaoLong Zhang,Hong Liu

Cell death & disease 14:525 PubMed37582769

2023

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen Jiang

Evidence-based complementary and alternative medicine : eCAM 2022:4026688 PubMed36588592

2022

Caudatin Inhibits the Proliferation, Invasion, and Glycolysis of Osteosarcoma Cells via the Wnt/- Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Zhizhong Sheng,Chi Su,Yuli Xia,Xiangfan Chen,Xiaobo Huang,Honglin Li,Changsheng Ma,Ligang Wang

Molecular therapy : the journal of the American Society of Gene Therapy 30:1054-1070 PubMed35038580

2022

Exosome-derived circTRPS1 promotes malignant phenotype and CD8+ T cell exhaustion in bladder cancer microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Siqi Wu,Zezhong Mou,Quan Zhou,Xiyu Dai,Yuxi Ou,Xinan Chen,Yiling Chen,Chenyang Xu,Yun Hu,Limin Zhang,Lujia Zou,Shengming Jin,Jimeng Hu,Shanhua Mao,Haowen Jiang

Journal of orthopaedic surgery and research 16:618 PubMed34663368

2021

MiR-4303 relieves chondrocyte inflammation by targeting ASPN in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyu Wang,Li Wang,Xingfa Guan,Changfeng Yue

EJNMMI research 11:59 PubMed34121134

2021

[Tc]Tc-Galacto-RGD integrin αβ-targeted imaging as a surrogate for molecular phenotyping in lung cancer: real-world data.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Fu,Yan Xie,Tong Fu,Fan Qiu,Fei Yu,Wei Qu,Xiaochen Yao,Aiping Zhang,Zhenhua Yang,Guoqiang Shao,Qingle Meng,Xiumin Shi,Yue Huang,Wei Gu,Feng Wang

Cancer cell international 21:292 PubMed34090440

2021

Long non-coding RNA LINC01116 is activated by EGR1 and facilitates lung adenocarcinoma oncogenicity via targeting miR-744-5p/CDCA4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Ren,Liang Chang,Xiaodong Hong,Lei Xing,Hong Zhang

Stem cells and development 30:587-600 PubMed33823616

2021

Study on the Safety of Human Oligodendrocyte Precursor Cell Transplantation in Young Animals and Its Efficacy on Myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Haipeng Zhou,Siliang Lu,Ke Li,Yinxiang Yang,Caiyan Hu,Zhaoyan Wang,Qian Wang,Ying He,Xiaohua Wang,Dou Ye,Qian Guan,Jing Zang,Chang Liu,Suqing Qu,Zuo Luan

Theranostics 11:4957-4974 PubMed33754038

2021

Single-cell transcriptome analysis of the heterogeneous effects of differential expression of tumor PD-L1 on responding TCR-T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renpeng Ding,Shang Liu,Shanshan Wang,Huanyi Chen,Fei Wang,Qumiao Xu,Linnan Zhu,Xuan Dong,Ying Gu,Xiuqing Zhang,Cheng-Chi Chao,Qianqian Gao

Journal of Cancer 12:1073-1084 PubMed33442405

2021

Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyuan Zhu,Haitao Huang,Shaorui Gu,Zhenchuan Liu,Xin Zhang,Kaiqin Wu,Tiancheng Lu,Lei Li,Chenglai Dong,Chongjun Zhong,Yongxin Zhou

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-Ki67 antibody [Ki-67]

Flow Cytometry (Intracellular) - PE Anti-Ki67 antibody [Ki-67] (AB270650)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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