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Rabbit Recombinant Monoclonal Ki67 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry (Intracellular) - PE Anti-Ki67 antibody [SP6] (AB282173), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/62500

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

Recommended products

Rabbit Recombinant Monoclonal Ki67 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP6

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - PE Anti-Ki67 antibody [SP6] (ab282173), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-Ki67 antibody [SP6] (ab282173)

    Flow cytometry overlay histogram showing wild-type Hap1 (green line) and MKI67 knockout Hap1 cells stained with ab282173 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum followed by the antibody (ab282173) (1x 106 in 100 μl at 0.008 μg/ml (1/62500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody (wild-type Hap1 - black line, MKI67 knockout Hap1- grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.This antibody can also be used in Hap1 cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com