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AB25205

PE Anti-KLRG1 antibody [2F1]

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(8 Publications)

Syrian hamster Monoclonal Klrg1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing Klrg1 protein.
1 Images
Flow Cytometry - PE Anti-KLRG1 antibody [2F1] (AB25205)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-KLRG1 antibody [2F1] (AB25205)

C57Bl/6 splenocytes were stained with APC Anti-Mouse NK1.1 and 0.25 ug PE Anti-Mouse KLRG1 (ab25205) (right panel) or 0.25 ug PE Golden Syrian Hamster IgG Isotype Control (left panel).

Key facts

Host species

Syrian hamster

Clonality

Monoclonal

Clone number

2F1

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLRG1 also known as killer cell lectin-like receptor G1 is a protein with a mass of about 30 kDa. It functions mechanically as an inhibitory receptor on natural killer (NK) cells and subsets of T cells. KLRG1 binds to classical cadherins like E-cadherin and N-cadherin modulating immune cell activity. Expression of KLRG1 is found mainly on mature NK cells and activated T cells particularly on those with an effector memory phenotype. Detecting KLRG1 expression often involves flow cytometry techniques using specific anti-KLRG1 antibodies.
Biological function summary

KLRG1 serves as a modulator of immune responses by delivering inhibitory signals that regulate immune cell cytotoxicity and cytokine production. It is not part of a larger protein complex but interacts with other molecules on the cell surface to perform its role. The engagement of its ligands mediates the reduction of immune responses which is important during chronic infections and cancer to prevent excessive immune activation.

Pathways

Several immune regulatory pathways involve KLRG1. It notably participates in the balance between immune activation and inhibition within the immune system. KLRG1 interacts with signaling pathways such as those regulated by T-cell receptor (TCR) signaling and NK cell activation. Related proteins in these pathways include CD226 and NKG2A which also play a role in determining the immune cell response.

KLRG1 has relevance in chronic infections and cancer due to its role in regulating immune cell activity. In chronic viral infections such as HIV and hepatitis KLRG1 expression is upregulated coinciding with T cell exhaustion. This marker is also significant in cancer where it contributes to the immune evasion mechanisms of tumors. The association with other proteins like PD-1 and TIM-3 further highlights KLRG1's function in these contexts serving as potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Klrg1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 31:107827 PubMed32610128

2020

The PD-1 Pathway Regulates Development and Function of Memory CD8 T Cells following Respiratory Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen E Pauken,Jernej Godec,Pamela M Odorizzi,Keturah E Brown,Kathleen B Yates,Shin Foong Ngiow,Kelly P Burke,Seth Maleri,Shannon M Grande,Loise M Francisco,Mohammed-Alkhatim Ali,Sabrina Imam,Gordon J Freeman,W Nicholas Haining,E John Wherry,Arlene H Sharpe

Journal of virology 88:14040-9 PubMed25253356

2014

Type I interferon signaling enhances CD8+ T cell effector function and differentiation during murine gammaherpesvirus 68 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan N Jennings,Jason M Grayson,Erik S Barton

Journal of immunology (Baltimore, Md. : 1950) 190:5020-9 PubMed23589620

2013

High viral burden restricts short-lived effector cell number at late times postinfection through increased natural regulatory T cell expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Amoah,Beth C Holbrook,Rama D Yammani,Martha A Alexander-Miller

Journal of immunology (Baltimore, Md. : 1950) 190:2178-87 PubMed23338237

2013

Critical role for all-trans retinoic acid for optimal effector and effector memory CD8 T cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

S Rameeza Allie,Weijun Zhang,Ching-Yi Tsai,Randolph J Noelle,Edward J Usherwood

Journal of immunology (Baltimore, Md. : 1950) 189:638-45 PubMed22706075

2012

Changes in functional but not structural avidity during differentiation of CD8+ effector cells in vivo after virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Amoah,Rama D Yammani,Jason M Grayson,Martha A Alexander-Miller

Journal of immunology (Baltimore, Md. : 1950) 188:3829-38 PubMed22422886

2012

CD27 stimulation promotes the frequency of IL-7 receptor-expressing memory precursors and prevents IL-12-mediated loss of CD8(+) T cell memory in the absence of CD4(+) T cell help.

Applications

Flow Cyt

Species

Unspecified reactive species

Han Dong,Nathan A Franklin,Drew J Roberts,Hideo Yagita,Martin J Glennie,Timothy N J Bullock

Journal of immunology (Baltimore, Md. : 1950) 186:6280-6 PubMed21525385

2011

Programmed death 1 regulates development of central memory CD8 T cells after acute viral infection.

Applications

Flow Cyt

Species

Mouse

S Rameeza Allie,Weijun Zhang,Shinchiro Fuse,Edward J Usherwood

Nature immunology 10:29-37 PubMed19043418

2008

Coregulation of CD8+ T cell exhaustion by multiple inhibitory receptors during chronic viral infection.

Applications

Flow Cyt

Species

Mouse

Shawn D Blackburn,Haina Shin,W Nicholas Haining,Tao Zou,Creg J Workman,Antonio Polley,Michael R Betts,Gordon J Freeman,Dario A A Vignali,E John Wherry
View all publications
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