AB95694

PE Anti-L1CAM antibody [5G3]

Name: PE Anti-L1CAM antibody [5G3] (ab95694) | Abcam
Brand: Abcam Limited
SKU: AB95694
Price: 260 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal L1CAM antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing L1CAM protein.

View Alternative Names

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

1 Images

Flow Cytometry - PE Anti-L1CAM antibody [5G3] (AB95694)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-L1CAM antibody [5G3] (AB95694)

Staining of the Panc-1 cell line with Mouse IgG2a kappa Isotype Control PE (open histogram) or ab95694 (filled histogram). Total viable cells were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G3

Isotype

IgG2a

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing L1CAM protein. The exact immunogen used to generate this antibody is proprietary information.

P32004

Epitope

Epitope recognised by ab95694 is in amino terminal Ig like domain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

ab95694 can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: BSA, PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.

Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

See full target information L1CAM

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:17261-75 PubMed25991672

2015

Peritoneal tumor spread in serous ovarian cancer-epithelial mesenchymal status and outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Auer,Anna Bachmayr-Heyda,Stefanie Aust,Nyamdelger Sukhbaatar,Agnes Teresa Reiner,Christoph Grimm,Reinhard Horvat,Robert Zeillinger,Dietmar Pils

PloS one 10:e0125145 PubMed25867515

2015

Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Abe E Kasonga,Vishwa Deepak,Marlena C Kruger,Magdalena Coetzee

PloS one 10:e0123684 PubMed25860483

2015

Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.

Applications

Flow Cyt

Species

Unspecified reactive species

Kaja Lund,Jennifer L Dembinski,Nina Solberg,Alfonso Urbanucci,Ian G Mills,Stefan Krauss

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-L1CAM antibody [5G3]

Flow Cytometry - PE Anti-L1CAM antibody [5G3] (AB95694)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise