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AB210264

PE Anti-LRRC32 antibody [GARP5]

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(1 Publication)

Mouse Monoclonal LRRC32 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Transfected cell line samples. Cited in 1 publication.

View Alternative Names

D11S833E, LRRC32, Transforming growth factor beta activator LRRC32, Garpin, Glycoprotein A repetitions predominant, Leucine-rich repeat-containing protein 32, GARP

1 Images
Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (AB210264)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (AB210264)

Flow cytometric analysis of untransfected (left) or LRRC32 transfected (right) cells labeling LRRC32 with 5 μl (0.5 μg) ab210264 (solid line), compared with 0.5 μg PE Mouse IgG1 isotype control (dashed line).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GARP5

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>The antibody has been diluted for use at 5 μl (0.5 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab210264 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRC32 also known as GARP (Glycoprotein A Repetitions Predominant) is a membrane protein with an approximate mass of 80 kDa. Scientists observe its expression mostly on regulatory T cells (Tregs) platelets and activated B cells. As an integral membrane protein LRRC32 plays an important role in cellular processes by anchoring latent transforming growth factor-beta (TGF-β) on the cell surface which is important for its regulation and activation.
Biological function summary

LRRC32 functions as part of a larger protein complex that regulates immune responses. By controlling the availability of TGF-β LRRC32 contributes directly to maintaining immune homeostasis and tolerance. Its presence on Tregs enables these cells to modulate immune responses effectively preventing excessive immune activity which could lead to autoimmune disorders. Hence LRRC32 serves as an important component in immune regulation through its interaction with and presentation of TGF-β.

Pathways

The activity of LRRC32 aligns with critical immune signaling pathways including the TGF-β signaling pathway and the FoxP3 regulatory pathway. In the TGF-β pathway it associates with other proteins like TGF-β receptor to mediate downstream signaling that influences cell growth differentiation and immune suppression. Additionally through its indirect interaction with the FoxP3 regulatory network LRRC32 aids in stabilizing Tregs and promoting their suppressive function within the immune system which involves the lineage-specific transcription factor FoxP3.

LRRC32 is relevant in conditions such as cancer and autoimmune diseases. Its association with TGF-β and FoxP3 makes it a potential target in cancer where excessive TGF-β activity might promote tumor progression and immune evasion. Conversely inadequate function of LRRC32 or TGF-β signaling could contribute to autoimmune diseases by failing to suppress harmful immune responses effectively. Its relation to GARP and TGF-β highlights its significant role in both the initiation and progression of these diseases direct implication in disease pathology and therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed : 19651619, PubMed : 19750484, PubMed : 22278742). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed : 22278742). Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (PubMed : 22278742). Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed : 19651619, PubMed : 19750484). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).
See full target information LRRC32
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 3:337-354 PubMed35256819

2022

An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Chan,Nicole Belmar,Sun Ho,Bryan Rogers,Marcia Stickler,Michelle Graham,Eileen Lee,Ninian Tran,Dong Zhang,Priyanka Gupta,Mien Sho,Tracy MacDonough,Andrew Woolley,Han Kim,Hong Zhang,Wei Liu,Pingping Zheng,Zoltan Dezso,Kyle Halliwill,Michele Ceccarelli,Susan Rhodes,Archana Thakur,Charles M Forsyth,Mengli Xiong,Siu Sze Tan,Ramesh Iyer,Marc Lake,Enrico Digiammarino,Li Zhou,Lance Bigelow,Kenton Longenecker,Russell A Judge,Cassie Liu,Max Trumble,Jonathan P Remis,Melvin Fox,Belinda Cairns,Yoshiko Akamatsu,Diane Hollenbaugh,Fiona Harding,Hamsell M Alvarez
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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