Mouse Monoclonal LRRC32 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Transfected cell line samples.
Images
![Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (AB210264), expandable thumbnail](https://content.abcam.com/products/images/pe-lrrc32-antibody-garp5-ab210264--flow-cytometry-img70319.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (AB210264)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Transfected cell line | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 5 µL for 105-108 Cells | Notes The antibody has been diluted for use at 5 μl (0.5 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl. |
Associated Products
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Target data
Function
Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed:19651619, PubMed:19750484, PubMed:22278742). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed:22278742). Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (PubMed:22278742). Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed:19651619, PubMed:19750484). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).
Alternative names
D11S833E, LRRC32, Transforming growth factor beta activator LRRC32, Garpin, Glycoprotein A repetitions predominant, Leucine-rich repeat-containing protein 32, GARP
Recommended products
Mouse Monoclonal LRRC32 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Transfected cell line samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- GARP5
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
ab210264 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LRRC32 also known as GARP (Glycoprotein A Repetitions Predominant) is a membrane protein with an approximate mass of 80 kDa. Scientists observe its expression mostly on regulatory T cells (Tregs) platelets and activated B cells. As an integral membrane protein LRRC32 plays an important role in cellular processes by anchoring latent transforming growth factor-beta (TGF-β) on the cell surface which is important for its regulation and activation.
- Biological function summary
LRRC32 functions as part of a larger protein complex that regulates immune responses. By controlling the availability of TGF-β LRRC32 contributes directly to maintaining immune homeostasis and tolerance. Its presence on Tregs enables these cells to modulate immune responses effectively preventing excessive immune activity which could lead to autoimmune disorders. Hence LRRC32 serves as an important component in immune regulation through its interaction with and presentation of TGF-β.
- Pathways
The activity of LRRC32 aligns with critical immune signaling pathways including the TGF-β signaling pathway and the FoxP3 regulatory pathway. In the TGF-β pathway it associates with other proteins like TGF-β receptor to mediate downstream signaling that influences cell growth differentiation and immune suppression. Additionally through its indirect interaction with the FoxP3 regulatory network LRRC32 aids in stabilizing Tregs and promoting their suppressive function within the immune system which involves the lineage-specific transcription factor FoxP3.
- Associated diseases and disorders
LRRC32 is relevant in conditions such as cancer and autoimmune diseases. Its association with TGF-β and FoxP3 makes it a potential target in cancer where excessive TGF-β activity might promote tumor progression and immune evasion. Conversely inadequate function of LRRC32 or TGF-β signaling could contribute to autoimmune diseases by failing to suppress harmful immune responses effectively. Its relation to GARP and TGF-β highlights its significant role in both the initiation and progression of these diseases direct implication in disease pathology and therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (ab210264), expandable thumbnail](https://content.abcam.com/products/images/pe-lrrc32-antibody-garp5-ab210264--flow-cytometry-img70319.jpg "Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (ab210264)")
Flow Cytometry - PE Anti-LRRC32 antibody [GARP5] (ab210264)
Flow cytometric analysis of untransfected (left) or LRRC32 transfected (right) cells labeling LRRC32 with 5 μl (0.5 μg) ab210264 (solid line), compared with 0.5 μg PE Mouse IgG1 isotype control (dashed line).
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