AB24884

PE Anti-Ly6g antibody [RB6-8C5]

Name: PE Anti-Ly6g antibody [RB6-8C5] (ab24884) | Abcam
Brand: Abcam Limited
SKU: AB24884
Price: 520 USD
Availability: InStock

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(12 Publications)

Rat Monoclonal Ly6g antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 12 publications.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

Unconjugated

Anti-Ly6g + Ly6c antibody [RB6-8C5]
660 APC

APC Anti-Ly6g antibody [RB6-8C5]
519 FITC

FITC Anti-Ly6g antibody [RB6-8C5]
778 PE/Cy7®

PE/Cy7® Anti-Ly6g antibody [RB6-8C5]
704 redFluor™ 710

redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]
450 violetFluor™ 450

violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RB6-8C5

Isotype

IgG2b

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Purified from TCS

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.142% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ly6g

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nitric oxide : biology and chemistry 98:50-59 PubMed32147582

2020

Effect of nitric oxide inhibition in Bacillus Calmette-Guerin bladder cancer treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yanina Verónica Langle,Natalia Patricia Balarino,Denise Belgorosky,Pablo Damián Cresta Morgado,Eduardo Omar Sandes,Lina Marino,Erica Rojas Bilbao,Macarena Zambrano,Catalina Lodillinsky,Ana María Eiján

Journal of immunological methods 197:139-50 PubMed8890901

1996

Flow cytometric identification of murine neutrophils and monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

E Lagasse,I L Weissman

Journal of virology 70:898-904 PubMed8551629

1996

Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea.

Applications

Unspecified application

Species

Unspecified reactive species

T M Tumpey,S H Chen,J E Oakes,R N Lausch

Journal of leukocyte biology 57:827-31 PubMed7790763

1995

Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes.

Applications

Unspecified application

Species

Unspecified reactive species

A L Rakhmilevich

Journal of immunology (Baltimore, Md. : 1950) 152:1836-46 PubMed8120393

1994

Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection.

Applications

Unspecified application

Species

Unspecified reactive species

C J Czuprynski,J F Brown,N Maroushek,R D Wagner,H Steinberg

The Journal of experimental medicine 179:259-68 PubMed8270870

1994

Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

J W Conlan,R J North

Journal of immunology (Baltimore, Md. : 1950) 151:2399-408 PubMed8360469

1993

Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.

Applications

Unspecified application

Species

Unspecified reactive species

T J Fleming,M L Fleming,T R Malek

The Journal of experimental medicine 178:151-61 PubMed7686211

1993

Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma.

Applications

Unspecified application

Species

Unspecified reactive species

A Stoppacciaro,C Melani,M Parenza,A Mastracchio,C Bassi,C Baroni,G Parmiani,M P Colombo

Journal of immunology (Baltimore, Md. : 1950) 147:22-8 PubMed1711076

1991

Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.

Applications

Unspecified application

Species

Unspecified reactive species

K Hestdal,F W Ruscetti,J N Ihle,S E Jacobsen,C M Dubois,W C Kopp,D L Longo,J R Keller

European journal of immunology 18:1819-26 PubMed2849552

1988

Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma.

Applications

Unspecified application

Species

Unspecified reactive species

M A Jutila,F G Kroese,K L Jutila,A M Stall,S Fiering,L A Herzenberg,E L Berg,E C Butcher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Ly6g antibody [RB6-8C5]

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise