AB77135

PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker

Name: PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (ab77135) | Abcam
Brand: Abcam Limited
SKU: AB77135
Price: 265 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal M6PR (cation independent) antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Virus within Human IGF2R.

View Alternative Names

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

1 Images

Flow Cytometry (Intracellular) - PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB77135)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB77135)

Flow cytometry analysis (intracellular staining) of human peripheral blood with anti-CD222 (MEM-238) PE.

Unconjugated

Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-238

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Virus within Human IGF2R. The exact immunogen used to generate this antibody is proprietary information.

P11717

Epitope

Ab77135 recognizes an epitope between domains 2 and 5 of CD222

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>Use 20 ul for 10^6 cells in a suspension or 100 ul of whole blood. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.

Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed : 18817523, PubMed : 2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed : 18817523, PubMed : 2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed : 18817523). This receptor also binds IGF2 (PubMed : 18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed : 10900005).

See full target information IGF2R

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 277:40575-82 PubMed12189157

2002

The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimír Leksa,Samuel Godár,Marek Cebecauer,Ivan Hilgert,Johannes Breuss,Ulrich H Weidle,Václav Horejsí,Bernd R Binder,Hannes Stockinger

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker

Flow Cytometry (Intracellular) - PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB77135)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise