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Mouse Monoclonal M6PR (cation independent) antibody - conjugated to PE. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Virus preparation containing IGF2R protein.

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Images

Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] (AB269311), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Virus preparation containing IGF2R protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11717

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytFlow Cyt (Intra)
Human
Tested
Expected

Tested
Tested

Species

Human

Dilution info

10 µL for 106 Cells

Notes

Or 100 μL of whole blood.

Expected
Expected

Species

Human

Dilution info

10 µL for 106 Cells

Notes

Or 100 μL of whole blood.

Associated Products

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Target data

Function

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed:18817523, PubMed:2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed:18817523, PubMed:2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed:18817523). This receptor also binds IGF2 (PubMed:18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed:10900005).

Alternative names

Recommended products

Mouse Monoclonal M6PR (cation independent) antibody - conjugated to PE. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Virus preparation containing IGF2R protein.

Key facts

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Virus preparation containing IGF2R protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11717
Clone number

MEM-240

Purification technique

Size-exclusion chromatography

Specificity

ab69311 recognizes an extracellular epitope between amino acids 698-1262.

Concentration
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Purification notes

The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.

Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

Associated diseases and disorders

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.

Product promise

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1 product image

  • Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] (ab269311), expandable thumbnail

    Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] (ab269311)

    Flow cytometry detection of M6PR (cation independent) in human peripheral blood labeling M6PR (cation independent) with ab269311.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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