Mouse Monoclonal M6PR (cation independent) antibody - conjugated to PE. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Virus preparation containing IGF2R protein.
Images
![Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] - Lysosome Membrane Marker (AB269311), expandable thumbnail](https://content.abcam.com/products/images/pe-m6pr-cation-independent-antibody-mem-240-lysosome-membrane-marker-ab269311--flow-cytometry-img98474.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] - Lysosome Membrane Marker (AB269311)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Virus preparation containing IGF2R protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11717
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Reactivity data
Select an application| Flow Cyt | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µL for 106 Cells | Notes Or 100 μL of whole blood. |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µL for 106 Cells | Notes Or 100 μL of whole blood. |
Associated Products
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Target data
Function
Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed:18817523, PubMed:2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed:18817523, PubMed:2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed:18817523). This receptor also binds IGF2 (PubMed:18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed:10900005).
Alternative names
CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R
Recommended products
Mouse Monoclonal M6PR (cation independent) antibody - conjugated to PE. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Virus preparation containing IGF2R protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Virus preparation containing IGF2R protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11717
- Clone number
- MEM-240
- Purification technique
- Size-exclusion chromatography
- Specificity
ab69311 recognizes an extracellular epitope between amino acids 698-1262.
- Concentration
- Purification notes
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.
- Biological function summary
The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.
- Pathways
M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.
- Associated diseases and disorders
M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] - Lysosome Membrane Marker (ab269311), expandable thumbnail](https://content.abcam.com/products/images/pe-m6pr-cation-independent-antibody-mem-240-lysosome-membrane-marker-ab269311--flow-cytometry-img98474.jpg "Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] - Lysosome Membrane Marker (ab269311)")
Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-240] - Lysosome Membrane Marker (ab269311)
M6PR (cation independent) Flow Cytometry staining using mouse Anti-M6PR (cation independent) antibody
Flow cytometry detection of M6PR (cation independent) in human peripheral blood labeling M6PR (cation independent) with ab269311.
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