Rabbit Monoclonal MelanA antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - PE Anti-MelanA antibody [EPR20380] (AB225499), expandable thumbnail](https://content.abcam.com/products/images/pe-melana-antibody-epr20380-ab225499--flow-cytometry-intracellular-img27470.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-MelanA antibody [EPR20380] (AB225499)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes The cellular localisation of this product has been verified in ICC/IF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
3 products for Alternative Version
Target data
Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Alternative names
MART1, MLANA, Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A
Recommended products
Rabbit Monoclonal MelanA antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20380
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-MelanA antibody [EPR20380] (ab225499), expandable thumbnail](https://content.abcam.com/products/images/pe-melana-antibody-epr20380-ab225499--flow-cytometry-intracellular-img27470.jpg "Flow Cytometry (Intracellular) - PE Anti-MelanA antibody [EPR20380] (ab225499)")
Flow Cytometry (Intracellular) - PE Anti-MelanA antibody [EPR20380] (ab225499)
Overlay histogram showing Malme-3M cells stained with ab225499 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225499, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in Malme-3M cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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