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AB246731

PE Anti-Melanoma gp100 antibody [EP4863(2)]

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(1 Publication)

Rabbit Monoclonal Melanoma gp100 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M

1 Images
Flow Cytometry (Intracellular) - PE Anti-Melanoma gp100 antibody [EP4863(2)] (AB246731)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Melanoma gp100 antibody [EP4863(2)] (AB246731)

Overlay histogram showing B16F10 cells stained with ab246731 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab246731) (1x 106 in 100μl at 0.1 μg/ml (1/5000)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in B16F10 cells fixed with 80 % methanol (5 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.

  • HRP

    HRP Anti-Melanoma gp100 antibody [EP4863(2)]

  • Biotin

    Biotin Anti-Melanoma gp100 antibody [EP4863(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Melanoma gp100 antibody [EP4863(2)]

  • 660 APC

    APC Anti-Melanoma gp100 antibody [EP4863(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Melanoma gp100 antibody [EP4863(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Melanoma gp100 antibody [EP4863(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Melanoma gp100 antibody [EP4863(2)]

  • Unconjugated

    Anti-Melanoma gp100 antibody [EP4863(2)]

  • Carrier free

    Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free

  • Carrier free

    Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4863(2)

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
See full target information PMEL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 13:17 PubMed38796460

2024

Mitotic MTH1 inhibitor TH1579 induces PD-L1 expression and inflammatory response through the cGAS-STING pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyu Shen,Emilio Guillén Mancina,Shenyu Chen,Theodora Manolakou,Helge Gad,Ulrika Warpman Berglund,Kumar Sanjiv,Thomas Helleday
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com